ProtParam
User-provided sequence:
10 20 30 40 50 60
MTQAEIVKCL REEYEIEHNK DQKPEKEDLP AITKKFYQRV LELLTIHKVP YSKQEDEETY
70 80 90 100 110 120
EEYLLSDTEE SGNKKKKQQG KLKNQDSDDE DVEARIASIK NKLRQKKGPT TERQQKRRES
130 140 150 160 170 180
KKLKRSKGVQ KLLLSSAKNL KNENVKHQKL KNGVVKSEQE TEDSKEQIQP VKVQPVYNQE
190 200 210 220 230 240
AKIVYSKVDF AANPGGKAKK SHQNPKEILK KLRDTKKHLT ELREQGETDK AAEIQTDIAW
250 260 270 280 290 300
RNAFDKVEGK KVKDDTKLLQ KAIKKRRVEK KKSKTKWTER KQKVEHDKEK RQKKRQENLE
310 320
KRSKDKKNRK LKTASKRGRI IPGY
References and
documentation are available.
Number of amino acids: 324
Molecular weight: 38134.67
Theoretical pI: 9.85
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 89
Atomic composition:
Carbon C 1663
Hydrogen H 2793
Nitrogen N 505
Oxygen O 513
Sulfur S 2
Formula: C
1663H
2793N
505O
513S
2
Total number of atoms: 5476
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.601, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.601, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.83
This classifies the protein as unstable.
Aliphatic index: 64.38
Grand average of hydropathicity (GRAVY): -1.535