ProtParam
User-provided sequence:
10 20 30 40 50 60
MTQEFIDKVS SYLTPDVDIA PISQGAEAIV FTTTTHPYLP RAKDSHQKYI IKYRPPKRYR
70 80 90 100 110 120
HPQIDQALTK HRTLNESRLL AKLYLIPGLC VPQLIACDPY NGFIWLEFLG EDLPGGHGFS
130 140 150 160 170 180
NLKNFLWMHD QDPYSDLVAT TLRKVGRQIG LLHWNDYCHG DLTSSNIVLV RDGARWTPHL
190 200 210 220 230 240
IDFGLGSVSN LVEDKGVDLY VLERAILSTH SKHAEKYNAW IMEGFEEVYR EQGAKGAKKL
250 260
KEVTKRFEEV RLRGRKRSML G
References and
documentation are available.
Number of amino acids: 261
Molecular weight: 29936.33
Theoretical pI: 8.81
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1353
Hydrogen H 2110
Nitrogen N 374
Oxygen O 381
Sulfur S 7
Formula: C
1353H
2110N
374O
381S
7
Total number of atoms: 4225
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45505
Abs 0.1% (=1 g/l) 1.520, assuming all pairs of Cys residues form cystines
Ext. coefficient 45380
Abs 0.1% (=1 g/l) 1.516, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.59
This classifies the protein as stable.
Aliphatic index: 89.27
Grand average of hydropathicity (GRAVY): -0.419