ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTRRYWNINL EEMMEAGVHF GHGTRKWNPK MAPYISAKRK GIHITNLTRT ARFLSEACDL 

        70         80         90        100        110        120 
VFDAASRGKQ FLIVGTKNKA ADSVEWAAIR ARCHYVNKKW LGGMLTNWST TETRLHKFRD 

       130        140        150        160        170        180 
LRMEQKTGRL NRLPKRDAAM LKRQLSRLQT YLGGIKYMTG VPDIVIIVDQ HEEYTALREC 

       190        200        210        220        230 
ITLGIPTICL TDTNCDPDLA DISIPANDDA ISSIRLILNK LVFAICEGRS SYIRNP 

References and documentation are available.

Number of amino acids: 236

Molecular weight: 26943.26

Theoretical pI: 9.59

Amino acid composition: 
Ala (A)  19	  8.1%
Arg (R)  21	  8.9%
Asn (N)  12	  5.1%
Asp (D)  13	  5.5%
Cys (C)   6	  2.5%
Gln (Q)   5	  2.1%
Glu (E)  11	  4.7%
Gly (G)  14	  5.9%
His (H)   6	  2.5%
Ile (I)  20	  8.5%
Leu (L)  22	  9.3%
Lys (K)  15	  6.4%
Met (M)   8	  3.4%
Phe (F)   6	  2.5%
Pro (P)   8	  3.4%
Ser (S)  11	  4.7%
Thr (T)  18	  7.6%
Trp (W)   5	  2.1%
Tyr (Y)   7	  3.0%
Val (V)   9	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1189
Hydrogen    H	      1916
Nitrogen    N	       348
Oxygen      O	       338
Sulfur      S	        14

Formula: C1189H1916N348O338S14
Total number of atoms: 3805

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38305
Abs 0.1% (=1 g/l)   1.422, assuming all pairs of Cys residues form cystines


Ext. coefficient    37930
Abs 0.1% (=1 g/l)   1.408, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.03
This classifies the protein as unstable.



Aliphatic index: 88.52

Grand average of hydropathicity (GRAVY): -0.324