ProtParam
User-provided sequence:
10 20 30 40 50 60
MTRTVSMSTA INEAMKISMR RDENVILIGE DVAGGAQVDH LQDDEAWGGV LGVTKGLVQE
70 80 90 100 110 120
FGRNRILDTP ISEAGYMGAA MAAAATGLRP IAELMFNDFI GSCLDQVLNQ GAKFRYMFGG
130 140 150 160 170 180
KAKVPVTVRT MHGAGFSAAA QHSQSLYALF TSIPGIKVVV PSTPYDAKGL LLAAIEDDDP
190 200 210 220 230 240
VIFFEDKTLY NMKGEVPEGY YTIPLGKADI KREGSDVTIV AIGKQVHTAL AAAEQLAKKG
250 260 270 280 290 300
LEVEVIDPRS LSPLDEDTIL SSVEKTNRLI VIDEANPRCS IATDIAAIVA DRGFDLLDAP
310 320 330 340
IKRITAPHTP VPFSPPLEKL YLPTPEKVIE TVSEMIGDQS LLHV
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 37007.53
Theoretical pI: 4.92
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1647
Hydrogen H 2647
Nitrogen N 435
Oxygen O 503
Sulfur S 13
Formula: C
1647H
2647N
435O
503S
13
Total number of atoms: 5245
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 17545
Abs 0.1% (=1 g/l) 0.474, assuming all pairs of Cys residues form cystines
Ext. coefficient 17420
Abs 0.1% (=1 g/l) 0.471, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.10
This classifies the protein as stable.
Aliphatic index: 98.98
Grand average of hydropathicity (GRAVY): 0.047