ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSAALINSG GSLPYSVSAG KAFPLGATVD DGGVNFALFS AHATGVELCL FDAQGKVEIQ 

        70         80         90        100        110        120 
RIALTEQTQQ IWHLYVHGLC AGQLYGYRVY GPYEPQLGHR FNPHKLLLDP YARQLVGRYH 

       130        140        150        160        170        180 
HHIANFGYEL DNPNEDLSFS TLDNAAYMPK CKVVDIRPLL AAAESQAIRP LSPHRNPLPI 

       190        200        210        220        230        240 
EQCIIYEMHL KGFTALHPEI DVPLRGTFAG LASKAAIDYL VKLGVNCVEL LPIQAFFSEP 

       250        260        270        280        290        300 
FLLEKQLSNY WGYNSIGFFA PEPSYLSSED IIEFRTMVDA LHGAGIEVIL DVVYNHSAEG 

       310        320        330        340        350        360 
SRLGPTFSFR GIDNLSYYRL HPNDKRFYIN DTGCGNTLNL NHPRMLQLVL DSLRYWVKVM 

       370        380        390        400        410        420 
GVDGFRFDLA ASLGREAYGF DPGSGFFDAL LQDPVLCRVK LIAEPWDIGP GGYQLGNFPV 

       430        440        450        460        470        480 
AFSEWNDRYR DTMRRFWRGD HGMLPEFARR FHGSGDFFEH SGRPPAASIN FLTSHDGFTL 

       490        500        510        520        530        540 
KDLVSYCERH NWANGEENRD GHHANFSYHY GVEGASNDVS VLVLRARQQR NLLTTLFLSQ 

       550        560        570        580        590        600 
GVPMLLSGDE TGRTQGGNNN AYCQDNPMNW FDWSSEGMDK HLLSFTQQLI ALRKRFPLLC 

       610        620        630        640        650        660 
AKRFIHEQLV ARSLESPSES PLGAAKFLTQ PSARLDWFSR QGKPMTKSLW SESMCRSLCV 

       670        680        690        700        710        720 
VLSGDLTAGI DVAGDNSFQA LLLMVNADEH PLAFTPPVFE HLGPWQCLLH TQTEPQYLTP 

       730        740        750 
TEPLDAQSSP TRYLLQDRSL MLFHADFIRN 

References and documentation are available.

Number of amino acids: 750

Molecular weight: 84191.39

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  56	  7.5%
Arg (R)  43	  5.7%
Asn (N)  35	  4.7%
Asp (D)  43	  5.7%
Cys (C)  13	  1.7%
Gln (Q)  31	  4.1%
Glu (E)  37	  4.9%
Gly (G)  60	  8.0%
His (H)  29	  3.9%
Ile (I)  27	  3.6%
Leu (L)  92	 12.3%
Lys (K)  19	  2.5%
Met (M)  15	  2.0%
Phe (F)  45	  6.0%
Pro (P)  45	  6.0%
Ser (S)  53	  7.1%
Thr (T)  29	  3.9%
Trp (W)  12	  1.6%
Tyr (Y)  29	  3.9%
Val (V)  37	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      3797
Hydrogen    H	      5752
Nitrogen    N	      1034
Oxygen      O	      1088
Sulfur      S	        28

Formula: C3797H5752N1034O1088S28
Total number of atoms: 11699

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   109960
Abs 0.1% (=1 g/l)   1.306, assuming all pairs of Cys residues form cystines


Ext. coefficient   109210
Abs 0.1% (=1 g/l)   1.297, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.08
This classifies the protein as stable.



Aliphatic index: 83.65

Grand average of hydropathicity (GRAVY): -0.219