ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSALRVLVC GDHPNLILYT SRFQHAKNIE FYLVNNSKNA NYEVSSLFYG TERFQIQNHF 

        70         80         90        100        110        120 
QSLLDLVDLN NENGGLVFDL IIMSASSLQE IPQVLRDIKP MMNKTTKILF ESSGFIYLEP 

       130        140        150        160        170        180 
FIKASVDLSL SNIFSIFTDY DIRRLDNGSY KQFTTANAKS FSVSIGQTTS VHENSYSSDI 

       190        200        210        220        230        240 
IPILNTFQKL FQKLFPRDVV TLYDHSPSAF LAKEWELALP QICFDPLLII LEEKNPSTLD 

       250        260        270        280        290        300 
DHVLAKPLIS GLLGESLLII KKMGIAMNNP NFQNEQTILK HWKNKCEDLP DGPALLYNFI 

       310        320        330        340        350        360 
HKASSLNIDL LLLQPILLAD DFGVKTPYLE CLFTMMTQYQ LLNKGDSEWF IRKDENTALT 

       370        380        390        400        410        420 
RVDDLQNSIA LKDGKIMQLQ NSESTLKNEI KELQSQVLSL KQEVSSSKAN NGQELEILKK 

       430        440        450        460        470        480 
KVQMGDNSLF DRPNSNTNGI SPSDNIVDVD LNYERSDQGN NSSGNDSRRQ SFFNSTSDTT 

       490        500        510        520        530        540 
LSRDETSLKE RELEVRMKEL ELQERELELQ RKALQQQQQY QQRPPKQVYS GPSGTPTSGN 

       550        560        570        580        590        600 
NNNKSYNPNR KSSYSQPQHV AMMTSRGLHG PSAASSSPVI SANNFVDPVS SGTPYSSNSS 

       610        620        630        640        650        660 
RFSQQIPSQQ YMHTVKPTSR KNRSSVMPNI GYVPGLTNNE YGRKFNGNGM NGTQSRLNSL 

       670        680        690        700        710        720 
SNQSTFRSQQ GPPITQQKSF QNNGGSMRTN RIPSANYNIS NQQSGFVNSI SSPNLSNLEN 

       730        740        750        760        770        780 
RNTVQNSRNA DSAPCVNQLN SDSPPQLQSL SQNGTSKVPQ INITQPSPIQ TNFATSDNPA 

       790        800        810        820        830 
AVIKLGTPSE DTVSAAATAN NISTMGDESR KEDVKEKKKK KFSFFGKRKK 

References and documentation are available.

Number of amino acids: 830

Molecular weight: 92872.08

Theoretical pI: 8.57

Amino acid composition: 
Ala (A)  33	  4.0%
Arg (R)  33	  4.0%
Asn (N)  80	  9.6%
Asp (D)  41	  4.9%
Cys (C)   5	  0.6%
Gln (Q)  59	  7.1%
Glu (E)  40	  4.8%
Gly (G)  39	  4.7%
His (H)  11	  1.3%
Ile (I)  47	  5.7%
Leu (L)  81	  9.8%
Lys (K)  53	  6.4%
Met (M)  18	  2.2%
Phe (F)  36	  4.3%
Pro (P)  44	  5.3%
Ser (S) 104	 12.5%
Thr (T)  45	  5.4%
Trp (W)   3	  0.4%
Tyr (Y)  22	  2.7%
Val (V)  36	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      4058
Hydrogen    H	      6444
Nitrogen    N	      1146
Oxygen      O	      1303
Sulfur      S	        23

Formula: C4058H6444N1146O1303S23
Total number of atoms: 12974

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49530
Abs 0.1% (=1 g/l)   0.533, assuming all pairs of Cys residues form cystines


Ext. coefficient    49280
Abs 0.1% (=1 g/l)   0.531, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.22
This classifies the protein as unstable.



Aliphatic index: 76.70

Grand average of hydropathicity (GRAVY): -0.620