ProtParam
User-provided sequence:
10 20 30 40 50 60
MTSASITNTG NETMNLPQMR SIWLDEDEEA EKLYGLQAQQ FMGSDDEENL GITFINSDKP
70 80 90 100 110 120
VLSNKKNIEL PPLSPNSHPS CHHRRSNSNS AKSKESSSSS SSANKTNHKK VFLKLNLLKK
130 140 150 160 170 180
KLLGAQPDIR GKGISTPFDF QHISHADTRN GFQDEQLQEP SSLSTEIKDD YTSSSSKRDS
190 200 210 220 230 240
KSLNKAFVTE RIPANRESKL ISRSHENKTS RLSVARSISV TSSNYSKNTQ GNNHSINGRV
250 260 270 280 290 300
VSTSTMATSI FEYSPNASPK QFKNKSHALG HRYTNSTDSS ESSLDFLKNY NFPTLLEDKP
310 320 330 340 350 360
ILDFLPRSQR SSAYRSLLET PNSNKDSAKA FFPSRQSPLP KRRNSIATPS PQSKFSYSDS
370 380
PVNHRKSFDD VLYSFNQLEP LQT
References and
documentation are available.
Number of amino acids: 383
Molecular weight: 42852.36
Theoretical pI: 9.48
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 1853
Hydrogen H 2939
Nitrogen N 545
Oxygen O 613
Sulfur S 6
Formula: C
1853H
2939N
545O
613S
6
Total number of atoms: 5956
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.441, assuming all pairs of Cys residues form cystines
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.441, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 64.57
This classifies the protein as unstable.
Aliphatic index: 60.13
Grand average of hydropathicity (GRAVY): -0.934