ProtParam
User-provided sequence:
10 20 30 40 50 60
MTSDALTIPS ELESALRLRT VQYFITKRPW LDLYGVHVRP VPPFGSTSRK PHFDPALIHR
70 80 90 100 110 120
CLPDELLFEV FARMMPYDLG RASCVCRKWR YTVRNPMFWR NACLKAWQTA GVIENYKILQ
130 140 150 160 170 180
SKYDGSWRKM WLLRSRVRTD GLYVSRNTYI RAGIAEWKIT NPVHIVCYYR YIRFYPSGRF
190 200 210 220 230 240
LYKNSSQKLK DVAKYMNFKA SKSENLYKGT YTLSMSDDKI EAAVLYPGTR PTVLRIRLRL
250 260 270 280 290 300
RGTAIGANNR MDLLSLVTSG VNDEEISSTE EDILGLVEGW EDDETHNPDI PAVSHKRGMT
310 320
AFVFVPFEEV DESVLNLPPE KMDYYVTG
References and
documentation are available.
Number of amino acids: 328
Molecular weight: 38042.79
Theoretical pI: 9.19
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1717
Hydrogen H 2665
Nitrogen N 467
Oxygen O 482
Sulfur S 15
Formula: C
1717H
2665N
467O
482S
15
Total number of atoms: 5346
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72560
Abs 0.1% (=1 g/l) 1.907, assuming all pairs of Cys residues form cystines
Ext. coefficient 72310
Abs 0.1% (=1 g/l) 1.901, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.27
This classifies the protein as unstable.
Aliphatic index: 82.59
Grand average of hydropathicity (GRAVY): -0.359