ProtParam
User-provided sequence:
10 20 30 40 50 60
MTSDPVNTNI SSPTLTDRNA DESWELLKRE FNTLFSNLKT DSKEEGNFTD NKGVIAKKPI
70 80 90 100 110 120
VLQDNDDSDF TQNQGKVATA TSTTSDRSFK RTLGSIEMKK RYVKKNCQAK FVFNTLEGKE
130 140 150 160 170 180
VCSKILQHTL GLLSLLLLTR KIRLLNFSSK LRLVIQQLSL FRYYLRFGNF AINLYKIIKR
190 200 210 220 230 240
FRWLREMKKL HYKDQSILFY FKNFRFFDII EAFYNLTDEL ILFHKLQSMF GKKNTSHANT
250 260 270 280 290 300
NRLMTFVKEQ HYILWEVLNI LAINKNIEQW RQLIRDEIYL SIYNTSGNAI KEYELKYKLP
310 320 330 340 350 360
TNDKVNLELR KNNITLDFYK IILNLLSNLI NIKGKRDKYN SELAYEIISV GSGVTELLKL
370
WNRAKVTSAN EHTSAV
References and
documentation are available.
Number of amino acids: 376
Molecular weight: 44131.00
Theoretical pI: 9.67
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2003
Hydrogen H 3184
Nitrogen N 538
Oxygen O 569
Sulfur S 7
Formula: C
2003H
3184N
538O
569S
7
Total number of atoms: 6301
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49975
Abs 0.1% (=1 g/l) 1.132, assuming all pairs of Cys residues form cystines
Ext. coefficient 49850
Abs 0.1% (=1 g/l) 1.130, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.45
This classifies the protein as unstable.
Aliphatic index: 97.23
Grand average of hydropathicity (GRAVY): -0.396