ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSIDNRPLP FIYQFTAGAI AGVSELLVMY PLDVVKTRMQ LQVTTKGHPA VVAAKAAVDH 

        70         80         90        100        110        120 
YTGVMDCLTK IVKKEGFSHL YKGITSPILM EAPKRAIKFS GNDTFQTFYK KIFPTPNGEM 

       130        140        150        160        170        180 
TQKIAIYSGA SAGAVEAFVV APFELVKIRL QDVNSQFKTP IEVVKNSVVK GGVLSLFNGL 

       190        200        210        220        230        240 
EATIWRHVLW NAGYFGIIFQ IRKLLPAAKT STEKTRNDLI AGAIGGTVGC LLNTPFDVVK 

       250        260        270        280        290        300 
SRIQRSSGPL RKYNWSLPSV LLVYREEGFK ALYKGFAPKV MRLAPGGGLL LVVFTNVMDF 

       310 
FREVKYGKKQ 

References and documentation are available.

Number of amino acids: 310

Molecular weight: 34206.24

Theoretical pI: 9.87

Amino acid composition: 
Ala (A)  25	  8.1%
Arg (R)  13	  4.2%
Asn (N)  11	  3.5%
Asp (D)   9	  2.9%
Cys (C)   2	  0.6%
Gln (Q)  10	  3.2%
Glu (E)  12	  3.9%
Gly (G)  26	  8.4%
His (H)   4	  1.3%
Ile (I)  19	  6.1%
Leu (L)  28	  9.0%
Lys (K)  27	  8.7%
Met (M)   8	  2.6%
Phe (F)  19	  6.1%
Pro (P)  16	  5.2%
Ser (S)  16	  5.2%
Thr (T)  20	  6.5%
Trp (W)   3	  1.0%
Tyr (Y)  11	  3.5%
Val (V)  31	 10.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1575
Hydrogen    H	      2498
Nitrogen    N	       408
Oxygen      O	       421
Sulfur      S	        10

Formula: C1575H2498N408O421S10
Total number of atoms: 4912

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33015
Abs 0.1% (=1 g/l)   0.965, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.962, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.26
This classifies the protein as stable.



Aliphatic index: 96.19

Grand average of hydropathicity (GRAVY): 0.120