ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSIGTGYDL SNSVFSPDGR NFQVEYAVKA VENGTTSIGI KCNDGVVFAV EKLITSKLLV 

        70         80         90        100        110        120 
PQKNVKIQVV DRHIGCVYSG LIPDGRHLVN RGREEAASFK KLYKTPIPIP AFADRLGQYV 

       130        140        150        160        170        180 
QAHTLYNSVR PFGVSTIFGG VDKNGAHLYM LEPSGSYWGY KGAATGKGRQ SAKAELEKLV 

       190        200        210        220        230        240 
DHHPEGLSAR EAVKQAAKII YLAHEDNKEK DFELEISWCS LSETNGLHKF VKGDLLQEAI 

       250        260        270        280 
DFAQKEINGD DDEDEDDSDN VMSSDDENAP VATNANATTD QEGDIHLE 

References and documentation are available.

Number of amino acids: 288

Molecular weight: 31536.12

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  24	  8.3%
Arg (R)   9	  3.1%
Asn (N)  15	  5.2%
Asp (D)  23	  8.0%
Cys (C)   3	  1.0%
Gln (Q)  10	  3.5%
Glu (E)  22	  7.6%
Gly (G)  25	  8.7%
His (H)   9	  3.1%
Ile (I)  16	  5.6%
Leu (L)  21	  7.3%
Lys (K)  21	  7.3%
Met (M)   3	  1.0%
Phe (F)  10	  3.5%
Pro (P)  10	  3.5%
Ser (S)  20	  6.9%
Thr (T)  13	  4.5%
Trp (W)   2	  0.7%
Tyr (Y)  10	  3.5%
Val (V)  22	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1388
Hydrogen    H	      2167
Nitrogen    N	       381
Oxygen      O	       447
Sulfur      S	         6

Formula: C1388H2167N381O447S6
Total number of atoms: 4389

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26025
Abs 0.1% (=1 g/l)   0.825, assuming all pairs of Cys residues form cystines


Ext. coefficient    25900
Abs 0.1% (=1 g/l)   0.821, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 22.40
This classifies the protein as stable.



Aliphatic index: 80.59

Grand average of hydropathicity (GRAVY): -0.464