ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSKLKRVAV AQLCSSADLT KNLKVVKELI SEAIQKKADV VFLPEASDYL SQNPLHSRYL 

        70         80         90        100        110        120 
AQKSPKFIRQ LQSSITDLVR DNSRNIDVSI GVHLPPSEQD LLEGNDRVRN VLLYIDHEGK 

       130        140        150        160        170        180 
ILQEYQKLHL FDVDVPNGPI LKESKSVQPG KAIPDIIESP LGKLGSAICY DIRFPEFSLK 

       190        200        210        220        230        240 
LRSMGAEILC FPSAFTIKTG EAHWELLGRA RAVDTQCYVL MPGQVGMHDL SDPEWEKQSH 

       250        260        270        280        290        300 
MSALEKSSRR ESWGHSMVID PWGKIIAHAD PSTVGPQLIL ADLDRELLQE IRNKMPLWNQ 


RRDDLFH 

References and documentation are available.

Number of amino acids: 307

Molecular weight: 34692.86

Theoretical pI: 6.37

Amino acid composition: 
Ala (A)  18	  5.9%
Arg (R)  17	  5.5%
Asn (N)   9	  2.9%
Asp (D)  22	  7.2%
Cys (C)   4	  1.3%
Gln (Q)  16	  5.2%
Glu (E)  19	  6.2%
Gly (G)  15	  4.9%
His (H)  10	  3.3%
Ile (I)  21	  6.8%
Leu (L)  37	 12.1%
Lys (K)  21	  6.8%
Met (M)   7	  2.3%
Phe (F)   8	  2.6%
Pro (P)  18	  5.9%
Ser (S)  28	  9.1%
Thr (T)   7	  2.3%
Trp (W)   5	  1.6%
Tyr (Y)   6	  2.0%
Val (V)  19	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1544
Hydrogen    H	      2471
Nitrogen    N	       429
Oxygen      O	       456
Sulfur      S	        11

Formula: C1544H2471N429O456S11
Total number of atoms: 4911

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36690
Abs 0.1% (=1 g/l)   1.058, assuming all pairs of Cys residues form cystines


Ext. coefficient    36440
Abs 0.1% (=1 g/l)   1.050, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.57
This classifies the protein as unstable.



Aliphatic index: 97.49

Grand average of hydropathicity (GRAVY): -0.335