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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSKPSTRND YLPRETHNGE YTGDSPEWQL QINITNKIGG INGDIWLSRD GRSVKWCIED 

        70         80         90        100        110        120 
QCLRQFTYNQ KIIKAGYIDF EKTPDCFVVV LSDIAHVYML KNGGSTTVCF PFQIGNAFWY 

       130        140        150        160        170        180 
ANGVILERET SASFMDGGYD LKPIEFDLKH KYITLTDPMA PFGLISITNT FKGGINSASG 

       190        200        210        220        230        240 
NKTDILQDFQ LVLFPSDKDK CIAVFLDRNS KVLRFYYSRI LSSDQSRKGE LTISSTKKTG 

       250        260        270        280        290        300 
LDAAGNSQKS GGISKDLRKF SHLTRRSTSI NSNSHDFNAA ERVLSGNVGN ASGRTDIFAL 

       310        320        330        340        350        360 
PSSCSRRSLS ATLDRMGNNI APTNRAAPSG FFDSSSANTA THSNITPVSQ PMQQQQQEYL 

       370        380        390        400        410        420 
NQTATSSKDI VLTEISSLKL PDDIIFTSRR LSSILSKLKF LSLRFERREG LLIFHEPTHF 

       430        440        450        460        470        480 
CKIWLIDLLP DVLDSIPFKI YGNSPQNMIR LENLKLKEPS RIQAMYIHEL LESCLILVSE 

       490        500        510        520        530        540 
GQNKEEYKAC LYDPFVKITS PSKNISEELT KQNSLPSLQK LFPYPETSFT KLCFEAVKYI 

       550        560        570        580        590        600 
TSPAFNISFI FLWQSAYSIL LSRANDDVVG GLKMEHDAFS LVLSLLILPI PSSSAQEYQE 

       610        620        630        640        650        660 
YKEIYERDLF QHLKQDSEIT SSVLPRIVIG LHLIREEYSL NVLCRNEHAL LGQFLRFATA 

       670        680        690        700        710        720 
AMGWPDLWQS YYVPKMDSES KTFLHPREQN STFFHPLDEP PSITKSLYSI TENSSIPLCP 

       730        740        750        760        770        780 
FISFSRLVAT DTQVELRITP RSFKILGLYE LVHSPNFLPD YVLGILSSFK VDKDELQTYP 

       790        800        810        820        830        840 
LGILVPLQNI LKILEDKLSE VRDNLELLDR ADLQRCSAII NSIRSDSKEV LKRGQRDSYM 

       850        860        870        880        890        900 
LCKVPLAKNR SSLSKKPSDI YSILSEIVKS ASQVPLDGSA MRMSNIQDDE DIDEGRSLKL 

       910        920        930        940        950        960 
NAGLIFSEDK RFTHVVSLLA YYRPTKTQFF TTKTEYAQIL AQKKYFAKIM ALRTCTNGVG 

       970        980        990       1000       1010       1020 
WGAVAYATEK PISTQKWVIQ PLNLISVFPD DTKITVKAPE DIAHDIVEWG QFHAGVSSGL 

      1030       1040       1050       1060       1070       1080 
RISKKATGIT GSWIAFNKPK ELDAYHGGFL LGLGLNGHLK NLEEWHIYNY LSPRNTHISI 

      1090       1100       1110       1120       1130       1140 
GLLLGMSSSM KGSMDSKLIK VISVHLVAFL PSGSSDLNID LKLQTAGIIG MGMLYLNSRH 

      1150       1160       1170       1180       1190       1200 
KRMSDSIFAQ LVSLLNVNDE MVADEEYRLA AGISLGLINL GAGQTKLRKW DSSLLGLGDD 

      1210       1220       1230       1240       1250       1260 
LPEDVYDSSD VEQNVMYEDL TTKLLEIVTS TYDVENDWIP ENSQIGAVIA IMFLFLKSNN 

      1270       1280       1290       1300       1310       1320 
FGISNMLKVD LKEILKANIN TRPELLMYRE WASNMILWEF IGDDLSFIMK DVDIGVKFSE 

      1330       1340       1350       1360       1370       1380 
LNTDLLPIYY TMAGRILAMG IRFASTGNLK IRNILLSLVD KFLPLYQYPG KQNLDFRLTI 

      1390       1400       1410       1420       1430       1440 
SVINVLTNVI VVSLSMVMCA SGDLEVLRRV KYLHEVASGP YSDLFQEIPS SKSDVSGVTQ 

      1450       1460       1470       1480       1490       1500 
VTSNTNTPGN SDRERVDETA ASLDDERSSN GSDISDPTAY LEDKKDIDDH YGKFISTNLA 

      1510       1520       1530       1540       1550       1560 
LGFLFLGSGQ YALNTSTLES IAFLSMSVLP TYTTPHPLQE LKHFWSIAVE PRCLVIKDIS 

      1570       1580       1590       1600       1610       1620 
TGDAVNNVPI ELVVEEDVEK EEVIREISTP CLLPDFSKIK SIRVKMHGYF PLEVNFTKDY 

      1630       1640       1650       1660       1670       1680 
SASDFFSGGT IIYIQRKSES VFENKASFRN VEDIHVALKR KAAESKNYSR LNLKNEQGNT 

      1690       1700       1710       1720       1730       1740 
TSSQLVESLG IQDLTMVELD TLLSAGNNTA LTDSESYNLG LLCSDKNSGD ILDCQLELWY 


KSFGPHDE


References and documentation are available. 

Number of amino acids: 1748

Molecular weight: 196143.82

Theoretical pI: 5.66


Amino acid composition: 
Ala (A)  85	  4.9%
Arg (R)  72	  4.1%
Asn (N)  90	  5.1%
Asp (D) 111	  6.4%
Cys (C)  20	  1.1%
Gln (Q)  59	  3.4%
Glu (E)  99	  5.7%
Gly (G)  95	  5.4%
His (H)  32	  1.8%
Ile (I) 133	  7.6%
Leu (L) 206	 11.8%
Lys (K) 108	  6.2%
Met (M)  35	  2.0%
Phe (F)  78	  4.5%
Pro (P)  72	  4.1%
Ser (S) 189	 10.8%
Thr (T)  95	  5.4%
Trp (W)  19	  1.1%
Tyr (Y)  60	  3.4%
Val (V)  90	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%



Total number of negatively charged residues (Asp + Glu): 210
Total number of positively charged residues (Arg + Lys): 180

Atomic composition:

Carbon      C	      8788
Hydrogen    H	     13856
Nitrogen    N	      2304
Oxygen      O	      2662
Sulfur      S	        55

Formula: C8788H13856N2304O2662S55
Total number of atoms: 27665

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   195150
Abs 0.1% (=1 g/l)   0.995, assuming all pairs of Cys residues form cystines


Ext. coefficient   193900
Abs 0.1% (=1 g/l)   0.989, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.68
This classifies the protein as unstable.



Aliphatic index: 95.43

Grand average of hydropathicity (GRAVY): -0.185