ProtParam
User-provided sequence:
10 20 30 40 50 60
MTSLTNAVFK RYLAVTPSAH QALKTRIKKK SSSFDKFFPQ QSNSRKKQWE TLNEDKASWF
70 80 90 100 110 120
KRKYAHVHAR EQDRAADPYG KKKAHVEKLK EIKNQAKLNQ KSHKSKFQNK DIALKLMNDN
130 140 150 160 170 180
PIFEYVYGTN SVYAALLNPS RNCHSRLLYH GTIPSKFLQI VDELKVTTEL VDKHRLNLLT
190 200 210 220 230 240
NYGVHNNIAL ETKPLQPVEI AYLGDMDTSS AALSIHELGF NNENIPHELP YGTKTDAKKF
250 260 270 280 290 300
PLGLYLDEIT DPHNIGAIIR SAYFLGVDFI VMSRRNCSPL TPVVSKTSSG ALELLPIFYV
310 320 330 340 350 360
DKPLEFFTKS QEMGGWTFIT SHLANATSEK YTVGKTISMH DLNGLCNELP VVLVVGNESQ
370 380 390 400 410
GVRTNLKMRS DFFVEIPFGG IEKGNRAPEP IVDSLNVSVA TALLIDNILT CK
References and
documentation are available.
Number of amino acids: 412
Molecular weight: 46387.22
Theoretical pI: 9.23
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 2086
Hydrogen H 3298
Nitrogen N 566
Oxygen O 608
Sulfur S 11
Formula: C
2086H
3298N
566O
608S
11
Total number of atoms: 6569
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37610
Abs 0.1% (=1 g/l) 0.811, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 0.805, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.21
This classifies the protein as stable.
Aliphatic index: 87.33
Grand average of hydropathicity (GRAVY): -0.365