ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSLYAPGAE DIRQRLRPFG FFFEKSLKDL IKGIRSHNET PEKLDQFFKQ VLSECREEVN 

        70         80         90        100        110        120 
SPDLNSKTNA VLKLTYLEMY GFDMAWCNFH ILEVMSSNKL QQKRVGYLAA SQSFYKDSDI 

       130        140        150        160        170        180 
LMLATNLLKK DLKYDGNNDV VKVGIALSGL STIITPSLAR DIADDLFTML NSTRPYIRKK 

       190        200        210        220        230        240 
AITALFKVFL QYPEALRDNF DKFVSKLDDD DISVVSAAVS VICELSKKNP QPFIQLSPLL 

       250        260        270        280        290        300 
YEILVTIDNN WIIIRLLKLF TNLSQVEPKL RAKLLPKILE LMESTVATSV IYESVNCIVK 

       310        320        330        340        350        360 
GNMLEEDDFE TAMACLERLH TFCDSQDPNL RYISCILFYK IGKINTDFIS RFDQLIIRLL 

       370        380        390        400        410        420 
SDVDVSIRSK AIELVEGIVD EDNLKAIVQT LMKQFVDEDV VILQTGSIVY EKSKRIPIII 

       430        440        450        460        470        480 
PENYKIKMVN VIISICSADN YSSVNDFEWY NAVIMDLAML CQDISDKSLG SKIGEQFRNL 

       490        500        510        520        530        540 
MIKVPSMREV TIANIIKLIS NDNINKQLPT VLRECIWCLG EFSTLVENGN DLIKIMTENI 

       550        560        570        580        590        600 
SYYSHSVQEV LILALVKVFS NWCNNFQEDK RFEIKMVLKE LIEFFENLSY SSTFEVQERS 

       610        620        630        640        650        660 
VEVLEFLRLS LEALEEDTEG LPMLLSEVLP SFFNAYELAP IARGTQLKLA VDENLDLETP 

       670        680        690        700        710        720 
FLTKEAADEL LDEQKSDAIS DLMSDISMDE QVELKFVDDS DTSYEEKEKL DDFENPFEIE 

       730        740        750        760        770        780 
REKERMSNPY YLGEEDEERT KNSKDLLDLN EEESSDKKPE TIRLNRTDNS LNSLSLSTTE 

       790        800        810        820        830        840 
ISRKKKKGKK KNRVQVLSDE PVIEAAPKRK DAFQKPHDNH STQNPLKKDK INLRMHSQLE 

       850        860        870        880        890        900 
NFDFSNFGQS SNAGRGSQEE GNLRKEDELE LSRLEANLIV KDEKDNLSDT EEVIVIKKKK 

       910        920        930 
KGKKSKSKNK LKTKAKNSPE PNEFLRDQST DI 

References and documentation are available.

Number of amino acids: 932

Molecular weight: 106924.31

Theoretical pI: 4.97

Amino acid composition: 
Ala (A)  40	  4.3%
Arg (R)  38	  4.1%
Asn (N)  59	  6.3%
Asp (D)  70	  7.5%
Cys (C)  12	  1.3%
Gln (Q)  31	  3.3%
Glu (E)  87	  9.3%
Gly (G)  25	  2.7%
His (H)   7	  0.8%
Ile (I)  71	  7.6%
Leu (L) 109	 11.7%
Lys (K)  85	  9.1%
Met (M)  22	  2.4%
Phe (F)  43	  4.6%
Pro (P)  30	  3.2%
Ser (S)  82	  8.8%
Thr (T)  38	  4.1%
Trp (W)   5	  0.5%
Tyr (Y)  23	  2.5%
Val (V)  55	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 157
Total number of positively charged residues (Arg + Lys): 123

Atomic composition:

Carbon      C	      4754
Hydrogen    H	      7626
Nitrogen    N	      1240
Oxygen      O	      1480
Sulfur      S	        34

Formula: C4754H7626N1240O1480S34
Total number of atoms: 15134

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62520
Abs 0.1% (=1 g/l)   0.585, assuming all pairs of Cys residues form cystines


Ext. coefficient    61770
Abs 0.1% (=1 g/l)   0.578, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.08
This classifies the protein as stable.



Aliphatic index: 96.73

Grand average of hydropathicity (GRAVY): -0.370