ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSNNSLLGR GRMSYSSTAP PRFKRSVDQR DTFSDNFDYD KDSSNRGRTY IAASNSTTGV 

        70         80         90        100        110        120 
PPPNNSRSGC TNNTNNTNNT SNTSNTNNND SVDENTVFET LPYYLPCFSW LPEYTFNKLW 

       130        140        150        160        170        180 
GDVIAGISVA SFQIPLALSY TTSIAHVPPL CGLYSLAISP FVYGILGSVP QMIVGPESAI 

       190        200        210        220        230        240 
SLVVGQAVES ITLHKENVSL IDISTVITFV SGTILLFSGI SRFGFLGNVL SKALLRGFIS 

       250        260        270        280        290        300 
SVGLVMIINS LISELKLDKF LVSLPQHYHT PFEKILFLID YAPAQYHIPT AIFSGCCLIV 

       310        320        330        340        350        360 
LFLTRLLKRK LMKYHKSAIF FPDILLVVIV TILISMKFNL KHRYGISIIG DFSMDNFDEL 

       370        380        390        400        410        420 
KNPLTRPRRK LIPDLFSASL IVAMLGFFES TTASKSLGTT YNLTVSSNRE LVALGFMNIV 

       430        440        450        460        470        480 
ISLFGALPAF GGYGRSKINA LSGAQSVMSG VFMGVITLIT MNLLLQFVHY IPNCVLSVIT 

       490        500        510        520        530        540 
TIIGISLLEE VPGDIKFHLR CGGFSELFVF AVTFCTTIFY SIEAGICIGC VYSIINIIKH 

       550        560        570        580        590        600 
SAKSRIQILA RVAGTSNFTN LDDYMMNMKR NSLDVEGTEE IEGCMIVRIP EPLTFTNSED 

       610        620        630        640        650        660 
LKQRLDRIER YGSSKIHPGR KSLRSKDSIK YVIFDLGGMT SIDSSAAQVL EEIITSYKRR 

       670        680        690        700        710        720 
NVFIYLVNVS INDKVRRRLF KAGVAASVER AQANNNENNT SNTFSDAGET YSPYFDSIDA 

       730        740        750 
ALYEIEKMKI KGNNVPNNDS ESFMSNTLFN SSLV 

References and documentation are available.

Number of amino acids: 754

Molecular weight: 83384.06

Theoretical pI: 8.56

Amino acid composition: 
Ala (A)  38	  5.0%
Arg (R)  34	  4.5%
Asn (N)  53	  7.0%
Asp (D)  31	  4.1%
Cys (C)  11	  1.5%
Gln (Q)  12	  1.6%
Glu (E)  30	  4.0%
Gly (G)  47	  6.2%
His (H)  11	  1.5%
Ile (I)  70	  9.3%
Leu (L)  75	  9.9%
Lys (K)  33	  4.4%
Met (M)  19	  2.5%
Phe (F)  46	  6.1%
Pro (P)  30	  4.0%
Ser (S)  85	 11.3%
Thr (T)  49	  6.5%
Trp (W)   2	  0.3%
Tyr (Y)  27	  3.6%
Val (V)  51	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      3755
Hydrogen    H	      5928
Nitrogen    N	       978
Oxygen      O	      1103
Sulfur      S	        30

Formula: C3755H5928N978O1103S30
Total number of atoms: 11794

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51855
Abs 0.1% (=1 g/l)   0.622, assuming all pairs of Cys residues form cystines


Ext. coefficient    51230
Abs 0.1% (=1 g/l)   0.614, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.07
This classifies the protein as unstable.



Aliphatic index: 99.66

Grand average of hydropathicity (GRAVY): 0.147