ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSRVSRLDR QVILIETGST QVVRNMAADQ MGDLAKQHPE DILSLLSRVY PFLLVKKWET 

        70         80         90        100        110        120 
RVTAARAVGG IVAHAPSWDP NESDLVGGTN EGSPLDNAQV KLEHEMKIKL EEATQNNQLN 

       130        140        150        160        170        180 
LLQEDHHLSS LSDWKLNEIL KSGKVLLASS MNDYNVLGKA DDNIRKQAKT DDIKQETSML 

       190        200        210        220        230        240 
NASDKANENK SNANKKSARM LAMARRKKKM SAKNTPKHPV DITESSVSKT LLNGKNMTNS 

       250        260        270        280        290        300 
AASLATSPTS NQLNPKLEIT EQADESKLMI ESTVRPLLEQ HEIVAGLVWQ FQGIYELLLD 

       310        320        330        340        350        360 
NLMSENWEIR HGAALGLREL VKKHAYGVSR VKGNTREENN LRNSRSLEDL ASRLLTVFAL 

       370        380        390        400        410        420 
DRFGDYVYDT VVAPVRESVA QTLAALLIHL DSTLSIKIFN CLEQLVLQDP LQTGLPNKIW 

       430        440        450        460        470        480 
EATHGGLLGI RYFVSIKTNF LFAHGLLENV VRIVLYGLNQ SDDDVQSVAA SILTPITSEF 

       490        500        510        520        530        540 
VKLNNSTIEI LVTTIWSLLA RLDDDISSSV GSIMDLLAKL CDHQEVLDIL KNKALEHPSE 

       550        560        570        580        590        600 
WSFKSLVPKL YPFLRHSISS VRRAVLNLLI AFLSIKDDST KNWLNGKVFR LVFQNILLEQ 

       610        620        630        640        650        660 
NPELLQLSFD VYVALLEHYK VKHTEKTLDH VFSKHLQPIL HLLNTPVGEK GKNYAMESQY 

       670        680        690        700        710        720 
ILKPSQHYQL HPEKKRSISE TTTDSDIPIP KNNEHINIDA PMIAGDITLL GLDVILNTRI 

       730        740        750        760        770        780 
MGAKAFALTL SMFQDSTLQS FFTNVLVRCL ELPFSTPRML AGIIVSQFCS SWLQKHPEGE 

       790        800        810        820        830        840 
KLPSFVSEIF SPVMNKQLLN RDEFPVFREL VPSLKALRTQ CQSLLATFVD VGMLPQYKLP 

       850        860        870        880        890        900 
NVAIVVQGET EAGPHAFGVE TAEKVYGEYY DKMFKSMNNS YKLLAKKPLE DSKHRVLMAI 

       910        920        930        940        950        960 
NSAKESAKLR TGSILANYAS SILLFDGLPL KLNPIIRSLM DSVKEERNEK LQTMAGESVV 

       970        980        990       1000       1010       1020 
HLIQQLLENN KVNVSGKIVK NLCGFLCVDT SEVPDFSVNA EYKEKILTLI KESNSIAAQD 

      1030       1040       1050       1060       1070       1080 
DINLAKMSEE AQLKRKGGLI TLKILFEVLG PSILQKLPQL RSILFDSLSD HENEEASKVD 

      1090       1100       1110       1120       1130       1140 
NEQGQKIVDS FGVLRALFPF MSDSLRSSEV FTRFPVLLTF LRSNLSVFRY SAARTFADLA 

      1150       1160       1170       1180       1190       1200 
KISSVEVMAY TIREILPLMN SAGSLSDRQG STELIYHLSL SMETDVLPYV IFLIVPLLGR 

      1210       1220       1230       1240       1250       1260 
MSDSNEDVRN LATTTFASII KLVPLEAGIA DPKGLPEELV ASRERERDFI QQMMDPSKAK 

      1270       1280       1290       1300       1310       1320 
PFKLPIAIKA TLRKYQQDGV NWLAFLNKYH LHGILCDDMG LGKTLQTICI IASDQYLRKE 

      1330       1340       1350       1360       1370       1380 
DYEKTRSVES RALPSLIICP PSLTGHWENE FDQYAPFLKV VVYAGGPTVR LTLRPQLSDA 

      1390       1400       1410       1420       1430       1440 
DIIVTSYDVA RNDLAVLNKT EYNYCVLDEG HIIKNSQSKL AKAVKEITAN HRLILTGTPI 

      1450       1460       1470       1480       1490       1500 
QNNVLELWSL FDFLMPGFLG TEKMFQERFA KPIAASRNSK TSSKEQEAGV LALEALHKQV 

      1510       1520       1530       1540       1550       1560 
LPFMLRRLKE DVLSDLPPKI IQDYYCELGD LQKQLYMDFT KKQKNVVEKD IENSEIADGK 

      1570       1580       1590       1600       1610       1620 
QHIFQALQYM RKLCNHPALV LSPNHPQLAQ VQDYLKQTGL DLHDIINAPK LSALRTLLFE 

      1630       1640       1650       1660       1670       1680 
CGIGEEDIDK KASQDQNFPI QNVISQHRAL IFCQLKDMLD MVENDLFKKY MPSVTYMRLD 

      1690       1700       1710       1720       1730       1740 
GSIDPRDRQK VVRKFNEDPS IDCLLLTTKV GGLGLNLTGA DTVIFVEHDW NPMNDLQAMD 

      1750       1760       1770       1780       1790       1800 
RAHRIGQKKV VNVYRIITKG TLEEKIMGLQ KFKMNIASTV VNQQNSGLAS MDTHQLLDLF 

      1810       1820       1830       1840       1850       1860 
DPDNVTSQDN EEKNNGDSQA AKGMEDIANE TGLTGKAKEA LGELKELWDP SQYEEEYNLD 


TFIKTLR 

References and documentation are available.

Number of amino acids: 1867

Molecular weight: 209976.56

Theoretical pI: 6.13

Amino acid composition: 
Ala (A) 120	  6.4%
Arg (R)  77	  4.1%
Asn (N) 103	  5.5%
Asp (D) 112	  6.0%
Cys (C)  16	  0.9%
Gln (Q)  87	  4.7%
Glu (E) 122	  6.5%
Gly (G)  83	  4.4%
His (H)  43	  2.3%
Ile (I) 112	  6.0%
Leu (L) 248	 13.3%
Lys (K) 136	  7.3%
Met (M)  49	  2.6%
Phe (F)  66	  3.5%
Pro (P)  75	  4.0%
Ser (S) 146	  7.8%
Thr (T)  92	  4.9%
Trp (W)  15	  0.8%
Tyr (Y)  44	  2.4%
Val (V) 121	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 234
Total number of positively charged residues (Arg + Lys): 213

Atomic composition:

Carbon      C	      9361
Hydrogen    H	     15045
Nitrogen    N	      2525
Oxygen      O	      2808
Sulfur      S	        65

Formula: C9361H15045N2525O2808S65
Total number of atoms: 29804

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   149060
Abs 0.1% (=1 g/l)   0.710, assuming all pairs of Cys residues form cystines


Ext. coefficient   148060
Abs 0.1% (=1 g/l)   0.705, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.66
This classifies the protein as unstable.



Aliphatic index: 100.42

Grand average of hydropathicity (GRAVY): -0.222