ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSSDSSVNT TSSSFGNISI SSPNHSSSTP PLNNGNGNNV SASETELKKH VLYSLQCLDE 

        70         80         90        100        110        120 
KTLTLKVKLD HLNKLVELKK SIPDLNKLGI SPTQLYKSVR PFIALPPKTI RTAGLRVMRY 

       130        140        150        160        170        180 
YLSNSNNVKE LLDLKVQYFI TRSLERDKHS EPERIQALKI IRTIMEIDCS LMPHCFVKGL 

       190        200        210        220        230        240 
VSIAENQEDN FCRVCLECLT EISIRNPQIS SHCGGIRTVF DAVLDPFYQG IQESLLICIL 

       250        260        270        280        290        300 
YLLSDKDTRI YIRPKSDLEI ILAPLTNSFN IGVKLKGASK EKEKEKEKED EVAMKKWTAS 

       310        320        330        340        350        360 
SKAVLTLIKS WIGIISLNSD DQGLKSVVDT LRMPQIELQE KALDSIFEIF RVQLPKSIQE 

       370        380        390        400        410        420 
TFGPQKATQT FNFGSETLQD LPSRTRSLRH NLLNNYLSVL LIAFIDNGLI EGLVYLGNYV 

       430        440        450        460        470        480 
ANRDGMSEQE KECSKNISLK STVLLAELLH MSNALLPPSQ CAKLQTLPSL VNSAISFRLD 

       490        500        510        520        530        540 
PRLRSSSNTM VTNLHSYSHN KSSTTLMDST LAIGLTGANK WRRIKGQDRR LDKVDDVKMK 

       550        560        570        580        590        600 
MEWHMDDNQF QQKIKDTQVL VTKDYQKWSW ELMFELLEGP LNNPQHLSNT LKTKFIKRIL 

       610        620        630        640        650        660 
SFLRPNKKLF STMAWTTENL KYVRTACVAL EVLISHEIGF DFLKDNKTII QIADMLKVEL 

       670        680        690        700        710        720 
DYNIKPPPSS SSSSENKKDN VRLLNPEKVL KTMSREYFTM LGTLSSNLLG LEILARNNIF 

       730        740        750        760        770        780 
DYIKPLAELP GRDDLSHLIM TSLDYNVNGA SRTILQKILT SSSRVVRYLA TKYLRFLLRS 

       790        800        810        820        830        840 
GVQDFSNWGV ELLVQQLNDV DAKVSALSLN VLDEACDDPS CLEVLIDLKP NLLKLGKPGK 

       850        860        870        880        890        900 
SLLLRFLSSP KGLENLLQNN GFVEQEEQLW ITSENATYVN AIESAVSESL SPSVWRFKEA 

       910        920        930        940        950        960 
PDGSSTSGVY LPPHFFGELA KTEKGCQLIR KSNNYQRFLK IIQDPTAKQL DKRASLIAIG 

       970        980        990       1000       1010       1020 
HIGSSVDGYS FVKESDTIKL LIGIAEKSQC LALRSTCFYA LGMISCIEEA QPIFNSFGWE 

      1030       1040       1050       1060       1070       1080 
SPSDLNSRIL LPKDLKNSTF LSVPQYQYQG SWADHSFETL PSNHFSDPIK NEIISFVGNL 

      1090       1100       1110       1120       1130       1140 
SSHITAEGAS KNLKRLKIKY PDHFATSEIL NAVFILLNTF KYRLGARRFI YDLFDVAIFS 


SDPYHDLN 

References and documentation are available.

Number of amino acids: 1148

Molecular weight: 129528.96

Theoretical pI: 8.58

Amino acid composition: 
Ala (A)  51	  4.4%
Arg (R)  49	  4.3%
Asn (N)  70	  6.1%
Asp (D)  61	  5.3%
Cys (C)  17	  1.5%
Gln (Q)  43	  3.7%
Glu (E)  66	  5.7%
Gly (G)  48	  4.2%
His (H)  21	  1.8%
Ile (I)  80	  7.0%
Leu (L) 155	 13.5%
Lys (K)  88	  7.7%
Met (M)  20	  1.7%
Phe (F)  46	  4.0%
Pro (P)  48	  4.2%
Ser (S) 125	 10.9%
Thr (T)  60	  5.2%
Trp (W)  12	  1.0%
Tyr (Y)  31	  2.7%
Val (V)  57	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 127
Total number of positively charged residues (Arg + Lys): 137

Atomic composition:

Carbon      C	      5792
Hydrogen    H	      9280
Nitrogen    N	      1550
Oxygen      O	      1732
Sulfur      S	        37

Formula: C5792H9280N1550O1732S37
Total number of atoms: 18391

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   113190
Abs 0.1% (=1 g/l)   0.874, assuming all pairs of Cys residues form cystines


Ext. coefficient   112190
Abs 0.1% (=1 g/l)   0.866, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.90
This classifies the protein as unstable.



Aliphatic index: 98.68

Grand average of hydropathicity (GRAVY): -0.236