ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSSFHFSHF SSLLSLFLFL FLIASHREHS KPFRKLIKKM YSKLNYSHQK GDQALKHPHL 

        70         80         90        100        110        120 
VRLQQSEVRG FTDMPNNGAS TSSAGSFARQ DSLTIAASLQ QRDRERHPVD FMETELDLGD 

       130        140        150        160        170        180 
YLQVLHDLDV PTDNVDFDDA ELQKCNILYD GEHPYEQPEL NGYERHVAYG TGYRVPGDYD 

       190        200        210        220        230        240 
QDGYKMNCEV KAETPDFGAT KTRRAVKRPV PYDDYQKEYS EESSDMTDND GSVDDSYFEP 

       250        260        270        280        290        300 
KSKKTKSAGL ENFKPQTRAR KYKLKADEEK AEPTYKLKRA RNNDAVRKSR KKAKELQDKK 

       310        320        330        340        350 
EAEHDKMKRR IAELEGLLQS ERDARRRDQD TLEQLLRNKG PMKEQRMPQR HILENFNK 

References and documentation are available.

Number of amino acids: 358

Molecular weight: 41724.61

Theoretical pI: 8.23

Amino acid composition: 
Ala (A)  22	  6.1%
Arg (R)  28	  7.8%
Asn (N)  14	  3.9%
Asp (D)  32	  8.9%
Cys (C)   2	  0.6%
Gln (Q)  19	  5.3%
Glu (E)  29	  8.1%
Gly (G)  16	  4.5%
His (H)  13	  3.6%
Ile (I)   6	  1.7%
Leu (L)  32	  8.9%
Lys (K)  35	  9.8%
Met (M)   9	  2.5%
Phe (F)  15	  4.2%
Pro (P)  16	  4.5%
Ser (S)  26	  7.3%
Thr (T)  15	  4.2%
Trp (W)   0	  0.0%
Tyr (Y)  16	  4.5%
Val (V)  13	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      1819
Hydrogen    H	      2858
Nitrogen    N	       536
Oxygen      O	       571
Sulfur      S	        11

Formula: C1819H2858N536O571S11
Total number of atoms: 5795

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23965
Abs 0.1% (=1 g/l)   0.574, assuming all pairs of Cys residues form cystines


Ext. coefficient    23840
Abs 0.1% (=1 g/l)   0.571, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.64
This classifies the protein as unstable.



Aliphatic index: 58.07

Grand average of hydropathicity (GRAVY): -1.146