ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSTNIDNRV PIEKTIEVIV KNDKGEEVKV IKRYQEYQIT VKRNRKVDER KKWKKFGECD 

        70         80         90        100        110        120 
NKTGLENTAY GDEQFLVLTR GAEVKEEEKD VVKCRICKKN HFTTKCPYKD ALELTTQPQK 

       130        140        150        160        170        180 
SEKEEKPISN KYIAPNLRGG YTGSAPSGSD VPSIMVSNLS QNATEKDLYE LFGQFGPVSR 

       190        200        210        220        230 
VSIPKSMEGS SKGFAYVTYN HLDSAEKALK QLNGHRYDYL VLSLEFAKKK SLN 

References and documentation are available.

Number of amino acids: 233

Molecular weight: 26531.17

Theoretical pI: 9.11

Amino acid composition: 
Ala (A)  10	  4.3%
Arg (R)  10	  4.3%
Asn (N)  14	  6.0%
Asp (D)  11	  4.7%
Cys (C)   4	  1.7%
Gln (Q)   8	  3.4%
Glu (E)  22	  9.4%
Gly (G)  14	  6.0%
His (H)   3	  1.3%
Ile (I)  11	  4.7%
Leu (L)  16	  6.9%
Lys (K)  31	 13.3%
Met (M)   3	  1.3%
Phe (F)   7	  3.0%
Pro (P)   9	  3.9%
Ser (S)  17	  7.3%
Thr (T)  14	  6.0%
Trp (W)   1	  0.4%
Tyr (Y)  11	  4.7%
Val (V)  17	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1171
Hydrogen    H	      1879
Nitrogen    N	       323
Oxygen      O	       364
Sulfur      S	         7

Formula: C1171H1879N323O364S7
Total number of atoms: 3744

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22140
Abs 0.1% (=1 g/l)   0.834, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.825, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.84
This classifies the protein as stable.



Aliphatic index: 70.64

Grand average of hydropathicity (GRAVY): -0.822