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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTSVKINHIS SLNEPYPSPS ALEAATTMHV QGAFVVATRK LPILKSGPID AMSERLGIPI

70 80 90 100 110 120
PEMIFGDNLV SITHRPTGWS IEFNAEDALE RVDKTGEKML QVAYAGEWSS SREKTSAGIS

130 140 150 160 170 180
EVVKPFDWSY TTDYCGTERP GSTGGAEGKE TKLHGDENTP DIPIELLKRR DPILFADEVV

190 200 210 220 230 240
LYESELDDNG CSIVSVKLRV MEHRMLLLCR MFMRLDNVLV RVRDTRVYVD FDKEEVIREY

250 260 270 280 290 300
TEREDKFENV KTKLFMQGLK LDQVTIALRD ANQVAPLLPL IKQGVESVIL GPGVATKTTT

310 320
TTRFPGQTQS TPQTQFVRPP PR


References and documentation are available.

Number of amino acids: 322 Molecular weight: 36042.21 Theoretical pI: 5.45
Amino acid composition: 
Ala (A) 17 5.3% Arg (R) 21 6.5% Asn (N) 9 2.8% Asp (D) 19 5.9% Cys (C) 3 0.9% Gln (Q) 10 3.1% Glu (E) 27 8.4% Gly (G) 20 6.2% His (H) 5 1.6% Ile (I) 18 5.6% Leu (L) 28 8.7% Lys (K) 18 5.6% Met (M) 10 3.1% Phe (F) 11 3.4% Pro (P) 21 6.5% Ser (S) 21 6.5% Thr (T) 27 8.4% Trp (W) 3 0.9% Tyr (Y) 7 2.2% Val (V) 27 8.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46 Total number of positively charged residues (Arg + Lys): 39 Atomic composition: Carbon C 1593 Hydrogen H 2555 Nitrogen N 435 Oxygen O 489 Sulfur S 13 Formula: C1593H2555N435O489S13 Total number of atoms: 5085 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 27055 Abs 0.1% (=1 g/l) 0.751, assuming all pairs of Cys residues form cystines Ext. coefficient 26930 Abs 0.1% (=1 g/l) 0.747, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 33.86 This classifies the protein as stable. Aliphatic index: 85.31 Grand average of hydropathicity (GRAVY): -0.337