ProtParam
User-provided sequence:
10 20 30 40 50 60
MTSVKINHIS SLNEPYPSPS ALEAATTMHV QGAFVVATRK LPILKSGPID AMSERLGIPI
70 80 90 100 110 120
PEMIFGDNLV SITHRPTGWS IEFNAEDALE RVDKTGEKML QVAYAGEWSS SREKTSAGIS
130 140 150 160 170 180
EVVKPFDWSY TTDYCGTERP GSTGGAEGKE TKLHGDENTP DIPIELLKRR DPILFADEVV
190 200 210 220 230 240
LYESELDDNG CSIVSVKLRV MEHRMLLLCR MFMRLDNVLV RVRDTRVYVD FDKEEVIREY
250 260 270 280 290 300
TEREDKFENV KTKLFMQGLK LDQVTIALRD ANQVAPLLPL IKQGVESVIL GPGVATKTTT
310 320
TTRFPGQTQS TPQTQFVRPP PR
References and
documentation are available.
Number of amino acids: 322
Molecular weight: 36042.21
Theoretical pI: 5.45
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1593
Hydrogen H 2555
Nitrogen N 435
Oxygen O 489
Sulfur S 13
Formula: C
1593H
2555N
435O
489S
13
Total number of atoms: 5085
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27055
Abs 0.1% (=1 g/l) 0.751, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.747, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.86
This classifies the protein as stable.
Aliphatic index: 85.31
Grand average of hydropathicity (GRAVY): -0.337