ProtParam
User-provided sequence:
10 20 30 40 50 60
MTTAVSPPPP QEQQLVHASQ TSRVSPKTVE SALNGLINWR SDKSKTEKPQ LLEEDELVYL
70 80 90 100 110 120
FVTLKKIPQK TRTNAYRIPL PHPLINPTVD SPEICLIIDD RPKSGLTKDD AMKKIKSENI
130 140 150 160 170 180
PITKVIKLSK LKSDYKAFEA KRKLCDSYDM FFTDRRIIPL LPRVIGKKFF TSKKIPVALD
190 200 210 220 230 240
LKHRNWKHQI EKACGSAMFF IRTGTCSVIK VGKLSMDICE ITENVMATLN GLVEFLPNKW
250 260 270 280 290 300
TYVRSLHLKL SESLALPIYQ SVPDLKLKID AFGSGKSVVQ EEKEGEVEEV VAVDGGEKSV
310 320 330 340 350 360
TGKGKKKKGR IHEVRYMDSN VAEVLDEDEI GGDEDNNEIV ESEEKKMKKR KKEVSEVAES
370 380 390 400 410 420
EKPMKKAAKG KLKPDVEKPM KKAAKGKSKP DVEKPMQKAA VKGKVKPDVV KLEKSKDGLK
430 440
AKKTTMVAKD ESGGGLKPKR KSVLRK
References and
documentation are available.
Number of amino acids: 446
Molecular weight: 49970.45
Theoretical pI: 9.57
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 91
Atomic composition:
Carbon C 2221
Hydrogen H 3692
Nitrogen N 608
Oxygen O 657
Sulfur S 17
Formula: C
2221H
3692N
608O
657S
17
Total number of atoms: 7195
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27180
Abs 0.1% (=1 g/l) 0.544, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.539, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.75
This classifies the protein as stable.
Aliphatic index: 82.78
Grand average of hydropathicity (GRAVY): -0.626