ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL 

        70         80         90        100        110        120 
EGDELENKTK KDNDKNLKFL KNKDSLVSNP HEIYGSMPLE QLIPIILRQR GPGFKFVDLN 

       130        140        150        160        170        180 
EKELQNEIKQ LGSDSSDGHN SEKKDTDGAD ENVQIGEDFM EVDYEDKDNP VDSRNETDHK 

       190        200        210        220        230        240 
TNENGETDDN IETVMTQEQF VKRRRDMLEH INLAMNESSL ALEFVSLLLS SVKESTGMSS 

       250        260        270        280        290        300 
MSPFLRKVVK PSSLNSDKIP YVAPTKKEYI ELDILNKGWK LQSLNESKDL LRASFNKLSS 

       310        320        330        340        350        360 
ILQNEHDYWN KIMQSISNKD VIFKIRDRTS GQKLLAIKYG YEDSGSTYKH DRGIANIRNN 

       370        380        390        400        410        420 
IESQNLDLIP HSSSVFKGTD FVHSVKKFLR VRIFTKIESE DDYILSGESV MDRDSESEEA 

       430        440        450        460        470        480 
ETKDIRKQIQ LLKKIIFEKE LMYQIKKECA LLISYGVSIE NENKVIIELP NEKFEIELLS 

       490        500        510        520        530        540 
LDDDSIVNHE QDLPKINDKR ANLMLVMLRL LLVVIFKKTL RSRISSPHGL INLNVDDDIL 

       550        560        570        580        590        600 
IIRPILGKVR FANYKLLLKK IIKDYVLDIV PGSSITETEV EREQPQENKN IDDENITKLN 

       610        620        630        640        650        660 
KEIRAFDKLL NIPRRELKIN LPLTEHKSPN LSLMLESPNY CNALIHIKFS AGTEANAVSF 

       670        680 
DTTFSDFKEV EDFLHFIVAE YIQQKKV 

References and documentation are available.

Number of amino acids: 687

Molecular weight: 78475.97

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  21	  3.1%
Arg (R)  27	  3.9%
Asn (N)  50	  7.3%
Asp (D)  55	  8.0%
Cys (C)   2	  0.3%
Gln (Q)  20	  2.9%
Glu (E)  63	  9.2%
Gly (G)  24	  3.5%
His (H)  15	  2.2%
Ile (I)  59	  8.6%
Leu (L)  77	 11.2%
Lys (K)  65	  9.5%
Met (M)  14	  2.0%
Phe (F)  24	  3.5%
Pro (P)  26	  3.8%
Ser (S)  66	  9.6%
Thr (T)  28	  4.1%
Trp (W)   2	  0.3%
Tyr (Y)  15	  2.2%
Val (V)  34	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 118
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      3463
Hydrogen    H	      5581
Nitrogen    N	       935
Oxygen      O	      1103
Sulfur      S	        16

Formula: C3463H5581N935O1103S16
Total number of atoms: 11098

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33475
Abs 0.1% (=1 g/l)   0.427, assuming all pairs of Cys residues form cystines


Ext. coefficient    33350
Abs 0.1% (=1 g/l)   0.425, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.44
This classifies the protein as unstable.



Aliphatic index: 94.61

Grand average of hydropathicity (GRAVY): -0.565