ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTTKPQNSKQ GLAEGEMDVS SLFKRTEVIG RGKFGVVYKG YNVKTGRVYA IKVLNLDSDS 

        70         80         90        100        110        120 
DEVEDVQREI QFLASLKQIS NITRYYGSYL KDTSLWIIME HCAGGSLRSL LRPGKIDEKY 

       130        140        150        160        170        180 
IGVIMRELLV ALKCIHKDNV IHRDIKAANV LITNEGNVKL CDFGVAAQVN QTSLRRQTMA 

       190        200        210        220        230        240 
GTPYWMAPEV IMEGVYYDTK VDIWSLGITT YEIATGNPPY CDVEALRAMQ LIIKSKPPRL 

       250        260        270        280        290        300 
EDRSYSTSLK EFIALCLDED PKERLSADDL LKSKFIRAHK ATPTSILKEL ISRYLLFRDK 

       310        320        330        340        350        360 
NKNKYKIEGS IPENEPSKPS EAPKPSQNGG GDEAQKSIAS NDNEIKRVNE GDVEMKWDFD 

       370        380        390        400        410        420 
SLSSSDYIIE NNINLDALAE DNNEWATAQH DLFNYAYPDE DSYYFDPTSH NTRPFVYQGT 

       430        440        450        460        470        480 
TIGKGYPGTI AQNSTLNAPV TNNYTNSKYP SKMVAGTTNT SGTHTAGPMT SSKRLESKAP 

       490        500        510        520        530        540 
KQLLELFEDN EIITAENDVN TEAPKISKSI SSLNAGNSSR DDFIPSISNE VNGNINNNKM 

       550        560        570        580        590        600 
RPHLPPLSSG NNYYSQSTPA LPLLQTKFNK TSKGPPTSGL TTAPTSIEIE IPEELPNSAL 

       610        620        630        640        650        660 
PTPASADPVL IPSTKARSST VTAGTPSSSS SIQYKSPSNV PRRLTVSNNR PEHCPSTITN 

       670        680        690        700        710        720 
QKLGSAVASN SGISSTPNNS NNYNNNTDSE NSRGSSGSNT ANSTQMGITN PGNVTKLSTH 

       730        740        750        760        770        780 
KASSPSRPLF GVGTSPNRKP AGSPTQNIGH NSTHTNLAPP PTMKPMANSK DNKDILLQPL 

       790        800        810        820        830        840 
NSIPSSSTLN TISGNSSNNL TSSNYFSNEK EGSRVNGDFK RNNPNLKLQM PLPTPVVRNK 

       850        860        870        880        890        900 
LLDPNTATSQ NNNGMPGSAG ISTNENINQF GFNTSSASNI PVSMTPISEK HIDFGGKIKR 

       910        920        930        940        950        960 
SQSISNRKNS SASEHPLNIL GSSVSGNVSG IGNNNVGSNN NSGPNNSVPL SANTGNTTIK 

       970        980        990       1000       1010       1020 
ANSTTIATSS SAAASTTAPI SQQTIPSGTQ FNHILSSAAT AANSVNSLGF GMCPPPQSLQ 

      1030       1040       1050       1060       1070       1080 
MEMFLDLESF LPGKQRRVDR KPQVLKELEN LLQMFEEGLP CIEHALKEQL ISTPIKDNEH 

References and documentation are available.

Number of amino acids: 1080

Molecular weight: 117061.52

Theoretical pI: 8.12

Amino acid composition: 
Ala (A)  64	  5.9%
Arg (R)  38	  3.5%
Asn (N) 109	 10.1%
Asp (D)  45	  4.2%
Cys (C)   8	  0.7%
Gln (Q)  35	  3.2%
Glu (E)  58	  5.4%
Gly (G)  67	  6.2%
His (H)  17	  1.6%
Ile (I)  69	  6.4%
Leu (L)  81	  7.5%
Lys (K)  68	  6.3%
Met (M)  22	  2.0%
Phe (F)  25	  2.3%
Pro (P)  77	  7.1%
Ser (S) 136	 12.6%
Thr (T)  82	  7.6%
Trp (W)   5	  0.5%
Tyr (Y)  29	  2.7%
Val (V)  45	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 106

Atomic composition:

Carbon      C	      5066
Hydrogen    H	      8101
Nitrogen    N	      1445
Oxygen      O	      1678
Sulfur      S	        30

Formula: C5066H8101N1445O1678S30
Total number of atoms: 16320

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71210
Abs 0.1% (=1 g/l)   0.608, assuming all pairs of Cys residues form cystines


Ext. coefficient    70710
Abs 0.1% (=1 g/l)   0.604, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.91
This classifies the protein as unstable.



Aliphatic index: 72.18

Grand average of hydropathicity (GRAVY): -0.610