ProtParam
User-provided sequence:
10 20 30 40 50 60
MTTKYIFVTG GVVSSLGKGI TAASLGRLLK NRGMKVTIQK FDPYINVDPG TMSPYQHGEV
70 80 90 100 110 120
FVTDDGAETD LDLGHYERFI DINLNKNSNV TTGKIYSSVL KKERRGDYLG GTVQVIPHVT
130 140 150 160 170 180
NEIKERVFRA GRETNADVVI TEIGGTVGDI ESLPFLEAIR QIKSDIGVDN VMYIHCTLIP
190 200 210 220 230 240
YLAAAGEMKS KPTQHSVKEL RSLGIQPNVI VVRTEKPVPE EMKEKIALFC DIRKDSVIEA
250 260 270 280 290 300
RDADTLYEVP LDLQAQNLDE IVCDHLNLSC QEADMTEWKS LVEKVKNLSG LVKIALVGKY
310 320 330 340 350 360
VALPDAYLSV AEALRHAGYA FDADINIKWV DSEDVTAENV AEQLQGVDGI LVPGGFGDRG
370 380 390 400 410 420
IEGKIEAIRY AREQKIPFLG ICLGMQLASI EFARNVLGLE GAHSAEINPD TPHPIIDLLP
430 440 450 460 470 480
EQKDVEDMGG TLRLGLYPCK LKNGTLAQSA YNDQVIYERH RHRYEFNNQY REQMEAKGFM
490 500 510 520 530
FSGTSPDGRL VEIVELGDHP FFIASQFHPE FVSRPTRPQP LFREFIQASL RK
References and
documentation are available.
Number of amino acids: 532
Molecular weight: 59254.60
Theoretical pI: 5.34
Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 2639
Hydrogen H 4185
Nitrogen N 717
Oxygen O 797
Sulfur S 17
Formula: C
2639H
4185N
717O
797S
17
Total number of atoms: 8355
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38195
Abs 0.1% (=1 g/l) 0.645, assuming all pairs of Cys residues form cystines
Ext. coefficient 37820
Abs 0.1% (=1 g/l) 0.638, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.03
This classifies the protein as stable.
Aliphatic index: 93.80
Grand average of hydropathicity (GRAVY): -0.242