ProtParam
User-provided sequence:
10 20 30 40 50 60
MTTMGEDEAP ALPKKSPLPE KIDEADVDDD PDDKVIKIVV VGDGASGKTS ICQRFAKESF
70 80 90 100 110 120
DKSYHQTLGL DFFSRRITLP HEMQVLVQVW DIGGQSIAGE MIDKYLTGAN IVFLVYDVTN
130 140 150 160 170 180
SKSFENAVDW LSVVKKNTKS SETPVKLVLM GNKTDLEERR VVSVEAHKNF ATSNDMMPTY
190 200 210 220 230 240
VSAKTGDTVF LTFRQAVAEV LNVGLSRAEV EADIEIVQGS VIEQPKQSDA SYARRSDQSR
STSVCSIT
References and
documentation are available.
Number of amino acids: 248
Molecular weight: 27318.83
Theoretical pI: 4.89
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1196
Hydrogen H 1916
Nitrogen N 322
Oxygen O 389
Sulfur S 9
Formula: C
1196H
1916N
322O
389S
9
Total number of atoms: 3832
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18575
Abs 0.1% (=1 g/l) 0.680, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.675, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.64
This classifies the protein as unstable.
Aliphatic index: 83.63
Grand average of hydropathicity (GRAVY): -0.293