ProtParam
User-provided sequence:
10 20 30 40 50 60
MTTNGFDPFE WRSFYFPGMS REEAHKLLGE PQVSIGTFLM RDSSRPGEYS LTVREADEGN
70 80 90 100 110 120
AVCHYLIERG EPKEDGTAAA GVKIANQSFP DIPALLNHFK MRVLTEASLL AAYKKPIIEV
130 140 150 160 170 180
VVGTFKFTGE RETDLPFEQG ERLEILSKTN QDWWEARNAL GTTGLVPANY VQIQMEFHND
190 200 210 220 230 240
RTSKGASQSS IGSSGGGAER FSSASTSSDN IELQPRLPAK AKVTFDRVPN AYDPTQLRVK
250 260 270
KGQTVLVTQK MSNGMYKAEL DGQIGSVPHT YLRFTAVSE
References and
documentation are available.
Number of amino acids: 279
Molecular weight: 30878.70
Theoretical pI: 5.83
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1365
Hydrogen H 2132
Nitrogen N 378
Oxygen O 424
Sulfur S 8
Formula: C
1365H
2132N
378O
424S
8
Total number of atoms: 4307
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.920, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.920, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.31
This classifies the protein as unstable.
Aliphatic index: 70.29
Grand average of hydropathicity (GRAVY): -0.476