ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTTRKTASSL QLLGKITGTK AGTKQKKMNF INGLIWLYMC VWMVHGKVTQ KDELKWNKGY 

        70         80         90        100        110        120 
SLPNLLEVTD QQKELSQWTL GDKVKLEEGR FVLTPGKNTK GSLWLKPEYS IKDAMTIEWT 

       130        140        150        160        170        180 
FRSFGFRGST KGGLAFWLKQ GNEGDSTELF GGSSKKFNGL MILLRLDDKL GESVTAYLND 

       190        200        210        220        230        240 
GTKDLDIESS PYFASCLFQY QDSMVPSTLR LTYNPLDNHL LKLQMDNRVC FQTRKVKFMG 

       250        260        270        280        290        300 
SSPFRIGTSA INDASKESFE ILKMKLYDGV IEDSLIPNVN PMGQPRVVTK VINSQTGEES 

       310        320        330        340        350        360 
FREKMPFSDK EESITSNELF EKMNKLEGKI MANDIDPLLR KMNKIVENER ELIQRLRPLL 

       370        380        390        400        410        420 
DLKKTAISDD SFQDFLSMNA NLDRLIKEQE KIRQDAKLYG KQTKGHDEIF SKISVWLALL 

       430        440 
IFIMITLAYY MFRINQDIKK VKLL 

References and documentation are available.

Number of amino acids: 444

Molecular weight: 50947.08

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  14	  3.2%
Arg (R)  18	  4.1%
Asn (N)  23	  5.2%
Asp (D)  27	  6.1%
Cys (C)   3	  0.7%
Gln (Q)  19	  4.3%
Glu (E)  28	  6.3%
Gly (G)  29	  6.5%
His (H)   3	  0.7%
Ile (I)  28	  6.3%
Leu (L)  54	 12.2%
Lys (K)  48	 10.8%
Met (M)  18	  4.1%
Phe (F)  22	  5.0%
Pro (P)  13	  2.9%
Ser (S)  33	  7.4%
Thr (T)  27	  6.1%
Trp (W)   8	  1.8%
Tyr (Y)  11	  2.5%
Val (V)  18	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2287
Hydrogen    H	      3656
Nitrogen    N	       602
Oxygen      O	       668
Sulfur      S	        21

Formula: C2287H3656N602O668S21
Total number of atoms: 7234

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60515
Abs 0.1% (=1 g/l)   1.188, assuming all pairs of Cys residues form cystines


Ext. coefficient    60390
Abs 0.1% (=1 g/l)   1.185, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.74
This classifies the protein as stable.



Aliphatic index: 86.94

Grand average of hydropathicity (GRAVY): -0.408