ProtParam
User-provided sequence:
10 20 30 40 50 60
MTTVSINKPN LLKFKHVKSF QPQEKDCGPV TSLNFDDNGQ FLLTSSSNDT MQLYSATNCK
70 80 90 100 110 120
FLDTIASKKY GCHSAIFTHA QNECIYSSTM KNFDIKYLNL ETNQYLRYFS GHGALVNDLK
130 140 150 160 170 180
MNPVNDTFLS SSYDESVRLW DLKISKPQVI IPSLVPNCIA YDPSGLVFAL GNPENFEIGL
190 200 210 220 230 240
YNLKKIQEGP FLIIKINDAT FSQWNKLEFS NNGKYLLVGS SIGKHLIFDA FTGQQLFELI
250 260 270 280 290 300
GTRAFPMREF LDSGSACFTP DGEFVLGTDY DGRIAIWNHS DSISNKVLRP QGFIPCVSHE
310 320
TCPRSIAFNP KYSMFVTADE TVDFYVYDE
References and
documentation are available.
Number of amino acids: 329
Molecular weight: 37122.08
Theoretical pI: 5.51
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1679
Hydrogen H 2548
Nitrogen N 424
Oxygen O 500
Sulfur S 14
Formula: C
1679H
2548N
424O
500S
14
Total number of atoms: 5165
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37860
Abs 0.1% (=1 g/l) 1.020, assuming all pairs of Cys residues form cystines
Ext. coefficient 37360
Abs 0.1% (=1 g/l) 1.006, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.42
This classifies the protein as stable.
Aliphatic index: 80.00
Grand average of hydropathicity (GRAVY): -0.212