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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTVNLDPDKV WIDGCFDFTH HGHAGAILQA RRTVSKENGK LFCGVHTDED IQHNKGTPVM

70 80 90 100 110 120
NSSERYEHTR SNRWCSEVVE AAPYVTDPNW MDKYQCQYVV HGDDITIDAN GEDCYKLVKE

130 140 150 160 170 180
MGRFKVVKRT YGVSTTEIIH RILTKKSLPP THPDYYPTTQ ELSFYSVAQD AVSKHCYVFQ

190 200 210 220 230 240
RDLDNVLVNG GYKFDAEDCV YVDGDFDLFH MGDIDQLRKL KMDLHPDKKL IVGITTSDYS

250 260 270 280 290 300
STIMTMKERV LSVLSCKYVD AVIIDADATS MSQYNCEKYH IGTAVLTAAG KFSEYLTKEL

310 320
IVKRVESQRE VYIARNQKKG MSI


References and documentation are available.

Number of amino acids: 323 Molecular weight: 36862.79 Theoretical pI: 6.00
Amino acid composition: 
Ala (A) 16 5.0% Arg (R) 14 4.3% Asn (N) 11 3.4% Asp (D) 29 9.0% Cys (C) 9 2.8% Gln (Q) 11 3.4% Glu (E) 17 5.3% Gly (G) 18 5.6% His (H) 13 4.0% Ile (I) 18 5.6% Leu (L) 19 5.9% Lys (K) 25 7.7% Met (M) 10 3.1% Phe (F) 10 3.1% Pro (P) 9 2.8% Ser (S) 20 6.2% Thr (T) 23 7.1% Trp (W) 3 0.9% Tyr (Y) 18 5.6% Val (V) 30 9.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46 Total number of positively charged residues (Arg + Lys): 39 Atomic composition: Carbon C 1627 Hydrogen H 2531 Nitrogen N 441 Oxygen O 499 Sulfur S 19 Formula: C1627H2531N441O499S19 Total number of atoms: 5117 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43820 Abs 0.1% (=1 g/l) 1.189, assuming all pairs of Cys residues form cystines Ext. coefficient 43320 Abs 0.1% (=1 g/l) 1.175, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.06 This classifies the protein as stable. Aliphatic index: 76.56 Grand average of hydropathicity (GRAVY): -0.441