ProtParam
User-provided sequence:
10 20 30 40 50 60
MTVNLDPDKV WIDGCFDFTH HGHAGAILQA RRTVSKENGK LFCGVHTDED IQHNKGTPVM
70 80 90 100 110 120
NSSERYEHTR SNRWCSEVVE AAPYVTDPNW MDKYQCQYVV HGDDITIDAN GEDCYKLVKE
130 140 150 160 170 180
MGRFKVVKRT YGVSTTEIIH RILTKKSLPP THPDYYPTTQ ELSFYSVAQD AVSKHCYVFQ
190 200 210 220 230 240
RDLDNVLVNG GYKFDAEDCV YVDGDFDLFH MGDIDQLRKL KMDLHPDKKL IVGITTSDYS
250 260 270 280 290 300
STIMTMKERV LSVLSCKYVD AVIIDADATS MSQYNCEKYH IGTAVLTAAG KFSEYLTKEL
310 320
IVKRVESQRE VYIARNQKKG MSI
References and
documentation are available.
Number of amino acids: 323
Molecular weight: 36862.79
Theoretical pI: 6.00
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1627
Hydrogen H 2531
Nitrogen N 441
Oxygen O 499
Sulfur S 19
Formula: C
1627H
2531N
441O
499S
19
Total number of atoms: 5117
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43820
Abs 0.1% (=1 g/l) 1.189, assuming all pairs of Cys residues form cystines
Ext. coefficient 43320
Abs 0.1% (=1 g/l) 1.175, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.06
This classifies the protein as stable.
Aliphatic index: 76.56
Grand average of hydropathicity (GRAVY): -0.441