ProtParam
User-provided sequence:
10 20 30 40 50 60
MTWILSTGMG PHEDKYAKHE RATFKKTYSS MKTLSLNHLT AKQHMLMALC RDISLLPPLT
70 80 90 100 110 120
YIFTSLRKAW RVSMRTSITL YEPQSLRDAF TYFWQKLNSA YDNNSSFEGA SQKAVNGDGK
130 140 150 160 170 180
DSLLLSALTT ARASEYLLCS LWCLVSLYLS YAILDSLMVR WIVKYSTVAA ILRMFSMSLI
190 200 210 220 230 240
IVTLELLLLS SLSPELDYFL HTWILISCVL TAVYIWQSYL TSDLRYIRNQ EGEVQEDTNV
250 260 270 280 290 300
PEETEDYEDG EDDADEDSHV VVADESTVDV PSNDSLSDNS DGGLFPVNRP SVSHSQSPKR
310 320 330 340 350 360
PKKYPKKAFN FTTKRTIDLY KITVLCVVPV GLASFITMLG LLRNLFIQRL DVEQLERILH
EMHPPA
References and
documentation are available.
Number of amino acids: 366
Molecular weight: 41673.81
Theoretical pI: 5.63
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1875
Hydrogen H 2938
Nitrogen N 482
Oxygen O 558
Sulfur S 16
Formula: C
1875H
2938N
482O
558S
16
Total number of atoms: 5869
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 64080
Abs 0.1% (=1 g/l) 1.538, assuming all pairs of Cys residues form cystines
Ext. coefficient 63830
Abs 0.1% (=1 g/l) 1.532, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.76
This classifies the protein as unstable.
Aliphatic index: 97.49
Grand average of hydropathicity (GRAVY): -0.092