ProtParam
User-provided sequence:
10 20 30 40 50 60
MTYGGRDQQY NKTNYKSRGG DFRGGRNSDR NSYNDRPQGG NYRGGFGGRS NYNQPQELIK
70 80 90 100 110 120
PNWDEELPKL PTFEKNFYVE HESVRDRSDS EIAQFRKENE MTISGHDIPK PITTFDEAGF
130 140 150 160 170 180
PDYVLNEVKA EGFDKPTGIQ CQGWPMALSG RDMVGIAATG SGKTLSYCLP GIVHINAQPL
190 200 210 220 230 240
LAPGDGPIVL VLAPTRELAV QIQTECSKFG HSSRIRNTCV YGGVPKSQQI RDLSRGSEIV
250 260 270 280 290 300
IATPGRLIDM LEIGKTNLKR VTYLVLDEAD RMLDMGFEPQ IRKIVDQIRP DRQTLMWSAT
310 320 330 340 350 360
WPKEVKQLAA DYLNDPIQVQ VGSLELSASH NITQIVEVVS DFEKRDRLNK YLETASQDNE
370 380 390 400 410 420
YKTLIFASTK RMCDDITKYL REDGWPALAI HGDKDQRERD WVLQEFRNGR SPIMVATDVA
430 440 450 460 470 480
ARGIDVKGIN YVINYDMPGN IEDYVHRIGR TGRAGATGTA ISFFTEQNKG LGAKLISIMR
490 500 510 520 530 540
EANQNIPPEL LKYDRRSYGG GHPRYGGGRG GRGGYGRRGG YGGGRGGYGG NRQRDGGWGN
RGRSNY
References and
documentation are available.
Number of amino acids: 546
Molecular weight: 60999.39
Theoretical pI: 8.90
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 2664
Hydrogen H 4185
Nitrogen N 795
Oxygen O 819
Sulfur S 17
Formula: C
2664H
4185N
795O
819S
17
Total number of atoms: 8480
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 76000
Abs 0.1% (=1 g/l) 1.246, assuming all pairs of Cys residues form cystines
Ext. coefficient 75750
Abs 0.1% (=1 g/l) 1.242, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.16
This classifies the protein as stable.
Aliphatic index: 69.47
Grand average of hydropathicity (GRAVY): -0.726