ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTYPVSAAAP ADISYSKNTP LVGLSKPPCL YQHASSSVDS FSSTFSDDDR SDLVAVPNES 

        70         80         90        100        110        120 
PHAFSYNPIS PNSLGVRLTI LRRSLEIMVN SPDILHELKK KAPVIAYPPS LRHTRNLTET 

       130        140        150        160        170        180 
ATLSASRDPL NGSLISPLVS NMPSPASRPV IQRATSLMVL PDNDTASKLN PAKSELENLL 

       190        200        210        220        230        240 
FLLNLALENN SFERASDLHM LSLLNIKKIN FDSDIQKSET LKKVLLDSLA EPFFENYKKF 

       250        260        270        280        290        300 
PHKDLGSKSQ YNEYEEKHDD IVSLADIKPQ QDYSRILHPF TSAKNSGPEA IFTCSQQYPW 

       310        320        330        340        350        360 
NFKAANDLAC LTFGISKNVI KALTLLDLIH TDSRNFVLEK IMNAEDDNQE IVFTGETIPI 

       370        380        390        400        410        420 
VQPNSTSNNN VPNLIWASLW AKRKNGLLVC VFEKTPCDYI DVMLNLRDFS VDSIIDTTHF 

       430        440        450        460        470        480 
LENFDKKKQQ ESTSPMTEKK TVKFANEIHD IGSVSHSLSK LIDDVRFGKV FSADDDLLPL 

       490        500        510        520        530        540 
SIRVANHVNE ERYFTLNCLS ENIPCAVTTS VLENEIKLKI HSLPYQAGLF IVDSHTLSLL 

       550        560        570        580        590        600 
SFNKSVAKNM FGLRLHELAG SSVTKLVPSL ADMISYINKT YPMLNITLPE NKGLVLTEHF 

       610        620        630        640        650        660 
FRKIEAEMHH DKDSFYTSIG LDGCHKDGNL IKVDVQLRVL NTNAVLLWIT HSRDVVIENY 

       670        680        690        700        710        720 
TTVPSQLPML KENEIDVVGS RGSSSASSKK SSEKIPVNTL KAMADLSISS AETISNSDDE 

       730        740        750        760        770        780 
VDLNQVNEKL RETSCGKVRG IESNDNNNYD DDMTMVDDPE LKHKIELTKM YTQDKSKFVK 

       790        800        810        820        830        840 
DDNFKVDEKF IMRIIEPING EEIKKETNEL DKRNSTLKAT YLTTPEANIG SQKRIKKFSD 

       850        860        870        880        890        900 
FTILQVMGEG AYGKVNLCIH NREHYIVVIK MIFKERILVD TWVRDRKLGT IPSEIQIMAT 

       910        920        930        940        950        960 
LNKNSQENIL KLLDFFEDDD YYYIETPVHG ETGSIDLFDV IEFKKDMVEH EAKLVFKQVV 

       970        980        990       1000       1010       1020 
ASIKHLHDQG IVHRDIKDEN VIVDSHGFVK LIDFGSAAYI KSGPFDVFVG TMDYAAPEVL 

      1030       1040       1050       1060       1070       1080 
GGSSYKGKPQ DIWALGVLLY TIIYKENPYY NIDEILEGEL RFDKSEHVSE ECISLIKRIL 

      1090       1100 
TREVDKRPTI DEIYEDKWLK I 

References and documentation are available.

Number of amino acids: 1101

Molecular weight: 124372.63

Theoretical pI: 5.48

Amino acid composition: 
Ala (A)  53	  4.8%
Arg (R)  36	  3.3%
Asn (N)  70	  6.4%
Asp (D)  83	  7.5%
Cys (C)  11	  1.0%
Gln (Q)  24	  2.2%
Glu (E)  75	  6.8%
Gly (G)  39	  3.5%
His (H)  31	  2.8%
Ile (I)  85	  7.7%
Leu (L) 112	 10.2%
Lys (K)  89	  8.1%
Met (M)  23	  2.1%
Phe (F)  45	  4.1%
Pro (P)  50	  4.5%
Ser (S) 103	  9.4%
Thr (T)  59	  5.4%
Trp (W)   7	  0.6%
Tyr (Y)  33	  3.0%
Val (V)  73	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 158
Total number of positively charged residues (Arg + Lys): 125

Atomic composition:

Carbon      C	      5549
Hydrogen    H	      8789
Nitrogen    N	      1461
Oxygen      O	      1707
Sulfur      S	        34

Formula: C5549H8789N1461O1707S34
Total number of atoms: 17540

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88295
Abs 0.1% (=1 g/l)   0.710, assuming all pairs of Cys residues form cystines


Ext. coefficient    87670
Abs 0.1% (=1 g/l)   0.705, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.34
This classifies the protein as stable.



Aliphatic index: 93.82

Grand average of hydropathicity (GRAVY): -0.319