ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVAGLRDFTL RTEDGSGKPV LDESNGEELM HVQTSVAVAL GNRPIESPGT LYITSRKLIW 

        70         80         90        100        110        120 
LSDVDMAKGY AVDFLSISLH AVSRDPEAYS SPCIYTQIEV EEDEDDESDS ESTEVLDLSK 

       130        140        150        160        170        180 
IREMRLVPSD STQLETLFDV FCECAELNPE PVQEEEEESG HNWVFSADQM DVRGGDDDAE 

       190        200        210        220 
WQISQSPTSV IGHSNGDEGL NQPMLELQIN DQRFEDAEEM VHESETKDH 

References and documentation are available.

Number of amino acids: 229

Molecular weight: 25602.86

Theoretical pI: 4.04

Amino acid composition: 
Ala (A)  11	  4.8%
Arg (R)   9	  3.9%
Asn (N)   7	  3.1%
Asp (D)  23	 10.0%
Cys (C)   3	  1.3%
Gln (Q)  10	  4.4%
Glu (E)  31	 13.5%
Gly (G)  13	  5.7%
His (H)   6	  2.6%
Ile (I)  10	  4.4%
Leu (L)  19	  8.3%
Lys (K)   5	  2.2%
Met (M)   7	  3.1%
Phe (F)   6	  2.6%
Pro (P)  10	  4.4%
Ser (S)  24	 10.5%
Thr (T)  11	  4.8%
Trp (W)   3	  1.3%
Tyr (Y)   4	  1.7%
Val (V)  17	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 14

Atomic composition:

Carbon      C	      1096
Hydrogen    H	      1697
Nitrogen    N	       293
Oxygen      O	       394
Sulfur      S	        10

Formula: C1096H1697N293O394S10
Total number of atoms: 3490

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22585
Abs 0.1% (=1 g/l)   0.882, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.877, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.02
This classifies the protein as unstable.



Aliphatic index: 75.72

Grand average of hydropathicity (GRAVY): -0.601