ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVASIRSGVL TLLHTACGAG ILAMPYAFKP FGLIPGVIMI VLCGACAMQS LFIQARVAKY 

        70         80         90        100        110        120 
VPQGRASFSA LTRLINPNLG IVFDLAIAIK CFGVGVSYMI VVGDLMPQIM SVWTRNAWLL 

       130        140        150        160        170        180 
NRNVQISLIM LFFVAPLSFL KKLNSLRYAS MVAISSVAYL CVLVLLHYVA PSDEILRLKG 

       190        200        210        220        230        240 
RISYLLPPQS HDLNVLNTLP IFVFAYTCHH NMFSIINEQR SSRFEHVMKI PLIAISLALI 

       250        260        270        280        290        300 
LYIAIGCAGY LTFGDNIIGN IIMLYPQAVS STIGRIAIVL LVMLAFPLQC HPARASIHQI 

       310        320        330        340        350        360 
LQHFAEENVS ISATSADEPT VATESSPLIR DSSLDLNEVI EEESIYQPKE TPLRGKSFIV 

       370        380        390        400        410        420 
ITCSILVASY LVAISVSSLA RVLAIVGATG STSISFILPG LFGYKLIGTE HKTAVPLTTK 

       430        440 
IFKYTGLLLF IWGLIIMITC LTAALKLN 

References and documentation are available.

Number of amino acids: 448

Molecular weight: 48840.34

Theoretical pI: 9.03

Amino acid composition: 
Ala (A)  41	  9.2%
Arg (R)  16	  3.6%
Asn (N)  15	  3.3%
Asp (D)   8	  1.8%
Cys (C)  10	  2.2%
Gln (Q)  12	  2.7%
Glu (E)  13	  2.9%
Gly (G)  25	  5.6%
His (H)  10	  2.2%
Ile (I)  52	 11.6%
Leu (L)  61	 13.6%
Lys (K)  14	  3.1%
Met (M)  14	  3.1%
Phe (F)  21	  4.7%
Pro (P)  21	  4.7%
Ser (S)  40	  8.9%
Thr (T)  22	  4.9%
Trp (W)   3	  0.7%
Tyr (Y)  15	  3.3%
Val (V)  35	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      2253
Hydrogen    H	      3618
Nitrogen    N	       560
Oxygen      O	       595
Sulfur      S	        24

Formula: C2253H3618N560O595S24
Total number of atoms: 7050

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39475
Abs 0.1% (=1 g/l)   0.808, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.795, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.99
This classifies the protein as unstable.



Aliphatic index: 130.18

Grand average of hydropathicity (GRAVY): 0.797