ProtParam
User-provided sequence:
10 20 30 40 50 60
MVAYSPIIAT YGNRAEQFLE TDSDFAKYHA KLNKKLQHLR SRCHLVTKDT KKYSSKNKYG
70 80 90 100 110 120
EINSEDYDNK TKLIGVLILL HAERDLALAE TLKLRARQRG KLKKSEEKVL STRLKKACKT
130 140 150 160 170 180
ADKLVNVTQN EQQWITRAQY LAFAKLVHSE YLINGKRFKR KDNAKISNNL ALVFAALEHL
190 200 210 220 230 240
KNLSLLAEEV VDNIVNKYQY SLKQYAGNLI TTPEINNFIV ERVQSDENKD DELVKLLLDN
250 260 270 280 290 300
GFNMKKITTS TEDQKVTTNI NWRSFNAKII DAEVAQFLEQ GLSIHPTQIT QYTQRLSKLE
310 320 330 340 350 360
KALDRHEFFI ANHDDQDDID EMVENSSENN QIILAYIKYN ILLTSISRER DLFTHLWNQW
370 380 390 400 410 420
LKLNTSLPSK LTKYKEMERI VKNLTKYLSD IMELPGVYSD DELLSQLDLC KLYFQLFLNT
430 440 450 460 470 480
GCLSVLYQSK GRYMEALALY VDAYRRLENK LSEIESLDEI LLPANLLSLN SVRSLQKRIE
490 500 510 520 530 540
NGGNSVITLA EYEKRNHGGS LGKYDLTVIE KLDSKKILPT DIQLKNLFPL KPKMLPIPSK
550 560 570 580 590
PTLFDLAFNY ITYDKQEPSA SQVKDSVTET ESISQTPISN EQTEGEPKKK RGFLGLFGR
References and
documentation are available.
Number of amino acids: 599
Molecular weight: 69006.04
Theoretical pI: 8.82
Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 87
Atomic composition:
Carbon C 3088
Hydrogen H 4961
Nitrogen N 833
Oxygen O 931
Sulfur S 11
Formula: C
3088H
4961N
833O
931S
11
Total number of atoms: 9824
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60990
Abs 0.1% (=1 g/l) 0.884, assuming all pairs of Cys residues form cystines
Ext. coefficient 60740
Abs 0.1% (=1 g/l) 0.880, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.60
This classifies the protein as stable.
Aliphatic index: 96.88
Grand average of hydropathicity (GRAVY): -0.509