ProtParam
User-provided sequence:
10 20 30 40 50 60
MVDAIQVAEL RRFVEQLKLN PSILHDPSLV FFKEYLRSLG AQVPKIEKTE RDYEDKAETK
70 80 90 100 110 120
PSFSPKHDDD DDDIMESDVE LDNSDVVEPD NEPPQPMGDP TAEVTDENRD DAQSEKSKAM
130 140 150 160 170 180
EAISDGRFDE AIEHLTKAVM LNPTSAILYA TRASVFLKVK KPNAAIRDAN VALQFNSDSA
190 200 210 220 230 240
KGYKSRGMAK AMLGQWEEAA ADLHVASKLD YDEEIGTMLK KVEPNAKRIE EHRRKYQRLR
250 260 270 280 290 300
KEKELQRAER ERRKQQEAQE REAQAALNDG EVISIHSTSE LEAKTKAAKK ASRLLILYFT
310 320 330 340 350 360
ATWCGPCRYM SPLYSNLATQ HSRVVFLKVD IDKANDVAAS WNISSVPTFC FIRDGKEVDK
370 380
VVGADKGSLE QKIAQHSSSK
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 42846.19
Theoretical pI: 5.50
Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 1866
Hydrogen H 2990
Nitrogen N 532
Oxygen O 599
Sulfur S 12
Formula: C
1866H
2990N
532O
599S
12
Total number of atoms: 5999
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30035
Abs 0.1% (=1 g/l) 0.701, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.698, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.20
This classifies the protein as unstable.
Aliphatic index: 75.29
Grand average of hydropathicity (GRAVY): -0.713