ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVDFLPKVTE INPPSEGNDG EDNIKPLSSG SEQRPLKEEG QQGGRRHHRR LSSMHEYFDP 

        70         80         90        100        110        120 
FSNAEVYYGP ITDPRKQSKI HRLNRTRTMS VFNKVSDFKN GMKDYTLKRR GSEDDSFLSS 

       130        140        150        160        170        180 
QGNRRFYIDN VDLALDELLA SEDTDKNHQI TIEDTGPKVI KVGTANSNGF KHVNVRGTYM 

       190        200        210        220        230        240 
LSNLLQELTI AKSFGRHQIF LDEARINENP VDRLSRLITT QFWTSLTRRV DLYNIAEIAR 

       250        260        270        280        290        300 
DSKIDTPGAK NPRIYVPYNC PEQYEFYIQA SQMNPSLKLE VEYLPKDITA EYVKSLNDTP 

       310        320        330        340        350        360 
GLLALAMEEH VNPSTGERSL VGYPYAVPGG RFNELYGWDS YLMALGLIES NKVDVARGMV 

       370        380        390        400        410        420 
EHFIFEIDHY SKILNANRSY YLCRSQPPFL TDMALLVFEK IGGKNNPNAI QLLKRAFRAA 

       430        440        450        460        470        480 
IKEYKEVWMS SPRLDSLTGL SCYHSDGIGI PPETEPDHFD TILLPYAEKY NVTLEKLRYL 

       490        500        510        520        530        540 
YNEGMIKEPK LDAFFLHDRA VRESGHDTTY RFEGVCAYLA TIDLNSLLYK YEKDIAFVIK 

       550        560        570        580        590        600 
EYFGNEYKDE NDGTVTDSEH WEELAELRKT RINKYMWDED SGFFFYYNTK LKCRTSYESA 

       610        620        630        640        650        660 
TTFWSLWAGL ATEEQAKITV EKALPQLEML GGLVACTEKS RGPISIDRPI RQWDYPFGWA 

       670        680        690        700        710        720 
PHQILAWKGL SAYGYQQVAT RLAYRWLYMI TKSFVDYNGM VVEKYDVTRG TDPHRVDAEY 

       730        740        750        760        770        780 
GNQGADFKGV ATEGFGWVNT SYLLGLKYMN NHARRALAAC SPPLPFFNSL KPSEKKLYYL 

References and documentation are available.

Number of amino acids: 780

Molecular weight: 89679.34

Theoretical pI: 6.05

Amino acid composition: 
Ala (A)  47	  6.0%
Arg (R)  46	  5.9%
Asn (N)  42	  5.4%
Asp (D)  47	  6.0%
Cys (C)   7	  0.9%
Gln (Q)  21	  2.7%
Glu (E)  58	  7.4%
Gly (G)  50	  6.4%
His (H)  18	  2.3%
Ile (I)  39	  5.0%
Leu (L)  74	  9.5%
Lys (K)  49	  6.3%
Met (M)  17	  2.2%
Phe (F)  34	  4.4%
Pro (P)  39	  5.0%
Ser (S)  51	  6.5%
Thr (T)  43	  5.5%
Trp (W)  12	  1.5%
Tyr (Y)  48	  6.2%
Val (V)  38	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 105
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      4034
Hydrogen    H	      6174
Nitrogen    N	      1078
Oxygen      O	      1196
Sulfur      S	        24

Formula: C4034H6174N1078O1196S24
Total number of atoms: 12506

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   137895
Abs 0.1% (=1 g/l)   1.538, assuming all pairs of Cys residues form cystines


Ext. coefficient   137520
Abs 0.1% (=1 g/l)   1.533, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.27
This classifies the protein as unstable.



Aliphatic index: 76.65

Grand average of hydropathicity (GRAVY): -0.544