ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVDLEQEFAL GGRCLAFHGP LMYEAKILKI WDPSSKMYTS IPNDKPGGSS QATKEIKPQK 

        70         80         90        100        110        120 
LGEDESIPEE IINGKCFFIH YQGWKSSWDE WVGYDRIRAY NEENIAMKKR LANEAKEAKK 

       130        140        150        160        170        180 
SLLEQQKKKK LSTSLGGPSN GGKRKGDSRS NASISKSTSQ SFLTSSVSGR KSGRSSANSL 

       190        200        210        220        230        240 
HPGSSLRSSS DQNGNDDRRR SSSLSPNMLH HIAGYPTPKI SLQIPIKLKS VLVDDWEYVT 

       250        260        270        280        290        300 
KDKKICRLPA DVTVEMVLNK YEHEVSQELE SPGSQSQLSE YCAGLKLYFD KCLGNMLLYR 

       310        320        330        340        350        360 
LERLQYDELL KKSSKDQKPL VPIRIYGAIH LLRLISVLPE LISSTTMDLQ SCQLLIKQTE 

       370        380        390        400 
DFLVWLLMHV DEYFNDKDPN RSDDALYVNT SSQYEGVALG M 

References and documentation are available.

Number of amino acids: 401

Molecular weight: 45203.43

Theoretical pI: 8.50

Amino acid composition: 
Ala (A)  17	  4.2%
Arg (R)  18	  4.5%
Asn (N)  16	  4.0%
Asp (D)  24	  6.0%
Cys (C)   6	  1.5%
Gln (Q)  18	  4.5%
Glu (E)  27	  6.7%
Gly (G)  25	  6.2%
His (H)   8	  2.0%
Ile (I)  22	  5.5%
Leu (L)  46	 11.5%
Lys (K)  37	  9.2%
Met (M)  10	  2.5%
Phe (F)   8	  2.0%
Pro (P)  18	  4.5%
Ser (S)  51	 12.7%
Thr (T)  12	  3.0%
Trp (W)   6	  1.5%
Tyr (Y)  16	  4.0%
Val (V)  16	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      1993
Hydrogen    H	      3180
Nitrogen    N	       548
Oxygen      O	       617
Sulfur      S	        16

Formula: C1993H3180N548O617S16
Total number of atoms: 6354

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57215
Abs 0.1% (=1 g/l)   1.266, assuming all pairs of Cys residues form cystines


Ext. coefficient    56840
Abs 0.1% (=1 g/l)   1.257, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.77
This classifies the protein as unstable.



Aliphatic index: 81.95

Grand average of hydropathicity (GRAVY): -0.585