ProtParam
User-provided sequence:
10 20 30 40 50 60
MVDRKAVIKN ADMSDDMQQD AIDCATQALE KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR
70 80
NFGSYVTHET KHFIYFYLGQ VAILLFKSG
References and
documentation are available.
Number of amino acids: 89
Molecular weight: 10343.86
Theoretical pI: 6.81
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 468
Hydrogen H 716
Nitrogen N 120
Oxygen O 135
Sulfur S 5
Formula: C
468H
716N
120O
135S
5
Total number of atoms: 1444
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 1.408, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 1.396, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.98
This classifies the protein as stable.
Aliphatic index: 77.87
Grand average of hydropathicity (GRAVY): -0.375