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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVDRKAVIKN ADMSDDMQQD AIDCATQALE KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR

70 80
NFGSYVTHET KHFIYFYLGQ VAILLFKSG


References and documentation are available.

Number of amino acids: 89 Molecular weight: 10343.86 Theoretical pI: 6.81
Amino acid composition: 
Ala (A) 8 9.0% Arg (R) 2 2.2% Asn (N) 4 4.5% Asp (D) 8 9.0% Cys (C) 2 2.2% Gln (Q) 4 4.5% Glu (E) 4 4.5% Gly (G) 4 4.5% His (H) 3 3.4% Ile (I) 8 9.0% Leu (L) 4 4.5% Lys (K) 10 11.2% Met (M) 3 3.4% Phe (F) 5 5.6% Pro (P) 1 1.1% Ser (S) 3 3.4% Thr (T) 4 4.5% Trp (W) 1 1.1% Tyr (Y) 6 6.7% Val (V) 5 5.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 12 Total number of positively charged residues (Arg + Lys): 12 Atomic composition: Carbon C 468 Hydrogen H 716 Nitrogen N 120 Oxygen O 135 Sulfur S 5 Formula: C468H716N120O135S5 Total number of atoms: 1444 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 14565 Abs 0.1% (=1 g/l) 1.408, assuming all pairs of Cys residues form cystines Ext. coefficient 14440 Abs 0.1% (=1 g/l) 1.396, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 21.98 This classifies the protein as stable. Aliphatic index: 77.87 Grand average of hydropathicity (GRAVY): -0.375