ProtParam
User-provided sequence:
10 20 30 40 50 60
MVDYIVEYDY DAVHDDELTI RVGEIIRNVK KLQEEGWLEG ELNGRRGMFP DNFVKEIKRE
70 80 90 100 110 120
TEPKDDNLPI KRERQGNVAS LVQRISTYGL PAGGIQPHPQ TKAIKKKTKK RQCKVLFDYS
130 140 150 160 170 180
PQNEDELELI VGDVIDVIEE VEEGWWSGTL NNKLGLFPSN FVKELESTED GETHNAQEES
190 200 210 220 230 240
EVPLTGPTSP LPSPGNGSEP APGSVAQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK
250 260 270 280 290 300
TEKPLILQPL GSRTQNVEVT KPDVDGKIKA KEYCRTLFPY TGTNEDELTF REGEIIHLIS
310 320 330 340 350 360
KETGEAGWWK GELNGKEGVF PDNFAVQISE LDKDFPKPKK PPPPAKGPAP KPDLSAAEKK
370 380 390 400 410 420
AFPLKAEEKD EKSLLEQKPS KPAAPQVPPK KPTAPTKASN LLRSPGAVYP KRPEKPVPPP
430 440 450 460 470 480
PPAAKINGEV SIISSKIDTE PVSKPKLDPE QLPVRPKSVD LDAFVARNSK ETDDVNFDDI
490 500 510 520 530 540
ASSENLLHLT ANRPKMPGRR LPGRFNGGHS PTQSPEKTLK LPKEDDSGNL KPLEFKKDAS
550 560 570 580 590 600
YSSKPSLSTP SSASKVNTAA FLTPLELKAK AEADDGKKNS VDELRAQIIE LLCIVDALKK
610 620 630
DHGKELEKLR KELEEEKAMR SNLEVEIAKL KKAVLLS
References and
documentation are available.
Number of amino acids: 637
Molecular weight: 70431.95
Theoretical pI: 6.07
Total number of negatively charged residues (Asp + Glu): 106
Total number of positively charged residues (Arg + Lys): 101
Atomic composition:
Carbon C 3126
Hydrogen H 5040
Nitrogen N 852
Oxygen O 978
Sulfur S 7
Formula: C
3126H
5040N
852O
978S
7
Total number of atoms: 10003
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41035
Abs 0.1% (=1 g/l) 0.583, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.581, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.13
This classifies the protein as unstable.
Aliphatic index: 75.78
Grand average of hydropathicity (GRAVY): -0.792