ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVEENSRVLI VLPYTPPSAT LQRIIGQTIP FLRECQSQLD IVIVPEFKTS FQLDSALGKM 

        70         80         90        100        110        120 
YSITRDVLLG YGMINSGINI IFNNIHFVES NLQWKVVLLP QESTFETWKL ELGQGQYHSI 

       130        140        150        160        170        180 
EHYALHDNIM EEIEGPKDAN KFHVTALGGT FDHIHDGHKI LLSVSTFITS QRLICGITCD 

       190        200        210        220        230        240 
ELLQNKKYKE LIEPYDTRCR HVHQFIKLLK PDLSVELVPL RDVCGPTGKV PEIECLVVSR 

       250        260        270        280        290        300 
ETVSGAETVN KTRIEKGMSP LAVHVVNVLG GREEDGWSEK LSSTEIRRLL KSSASPTCTP 


QNPCV 

References and documentation are available.

Number of amino acids: 305

Molecular weight: 34306.52

Theoretical pI: 5.89

Amino acid composition: 
Ala (A)   8	  2.6%
Arg (R)  13	  4.3%
Asn (N)  12	  3.9%
Asp (D)  12	  3.9%
Cys (C)   8	  2.6%
Gln (Q)  13	  4.3%
Glu (E)  25	  8.2%
Gly (G)  19	  6.2%
His (H)  11	  3.6%
Ile (I)  25	  8.2%
Leu (L)  33	 10.8%
Lys (K)  17	  5.6%
Met (M)   5	  1.6%
Phe (F)  10	  3.3%
Pro (P)  16	  5.2%
Ser (S)  23	  7.5%
Thr (T)  20	  6.6%
Trp (W)   3	  1.0%
Tyr (Y)   7	  2.3%
Val (V)  25	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1531
Hydrogen    H	      2445
Nitrogen    N	       411
Oxygen      O	       455
Sulfur      S	        13

Formula: C1531H2445N411O455S13
Total number of atoms: 4855

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27430
Abs 0.1% (=1 g/l)   0.800, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   0.785, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 64.24
This classifies the protein as unstable.



Aliphatic index: 100.56

Grand average of hydropathicity (GRAVY): -0.130