ProtParam
User-provided sequence:
10 20 30 40 50 60
MVEGWRNGFR DATNSKPLFV TIYATVIIGV LVSSFYVFSA IYSPTNGSSS FLSFPPLSTS
70 80 90 100 110 120
GRIHSLPQEN ATLELPVAPP PPPQALPPPV LEEAQGNSLG KIWVSPPRDK KMPPLETFKL
130 140 150 160 170 180
TKELFGERVK DNVIIVTFGN YAFMDFILTW VKHLTDLDLS NILVGAMDTK LLEALYWKGV
190 200 210 220 230 240
PVFDMGSHMS TVDVGWGSPT FHKMGREKVI LIDSVLPFGY ELLMCDTDMV WLKNPMPYLA
250 260 270 280 290 300
RFPDADVLTS SDQVVPTVID DSLDIWQQVG AAYNIGIFHW RPTESAKKLA KEWKEILLAD
310 320 330 340 350 360
DKVWDQNGFN EIVRRQLGPS VEGDSGLFYA YDGNLKVGIL PASIFCSGHT YFVQAMYQQL
370 380 390 400 410 420
RLEPYAVHTT FQYAGTEGKR HRLREGMVFY DPPEYYDSPG GFIAFKPSIP KSLLLDGKHT
430 440 450 460 470 480
IESHFILVNH QMKQIRSALA IASLLNRTLV MPPIWCRLDR LWFGHPGTLQ GSMTRQPFIC
490 500 510 520 530 540
PLDHVFEVNI MLKELPEEEF GPGIGIREYS FLDNPLLPKQ VKESWLDVQL CQEGKEGCEA
550 560 570 580 590 600
SNNTSPSRVL KFPKRSNEDT FKAIFSSFDD VKVIKFSSIE DAFIGFSDKE REERFRRRVK
610 620 630 640
RYVGIWCCEE NKTPGHIYYD MYWDEKPGWK PVPPQTPEED HPPL
References and
documentation are available.
Number of amino acids: 644
Molecular weight: 73213.08
Theoretical pI: 5.69
Total number of negatively charged residues (Asp + Glu): 79
Total number of positively charged residues (Arg + Lys): 66
Atomic composition:
Carbon C 3352
Hydrogen H 5107
Nitrogen N 853
Oxygen O 941
Sulfur S 25
Formula: C
3352H
5107N
853O
941S
25
Total number of atoms: 10278
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 121280
Abs 0.1% (=1 g/l) 1.657, assuming all pairs of Cys residues form cystines
Ext. coefficient 120780
Abs 0.1% (=1 g/l) 1.650, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.90
This classifies the protein as unstable.
Aliphatic index: 83.51
Grand average of hydropathicity (GRAVY): -0.233