ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVEKDTWKLI TATALFTVAV TTITDYAWTS WQAQKQVIAQ QKNKNKGGQT KSDTDKYHQY 

        70         80         90        100        110        120 
DEQFIRQSLK NNVEFLGEDT IEKLSNQYVV VVGAGGVGSW VVNSLVRSGC RKIRVVDFDQ 

       130        140        150        160        170        180 
VSLSSLNRHS CAILNDVGTP KVECLRRHMR EIAPWCEIDP INELWTLQNG ERLTLGNGTP 

       190        200        210        220        230        240 
DFIVDCIDNI DTKVDLLEFA YNHGIKVISS MGASAKSDPT KLNVGDLATT EEDPLARVVR 

       250        260        270        280        290        300 
RKLKKRGILS GIPVVFSAEK PDPKKAKLLP LPDEEYERGK VDELSALKDF RVRILPVLGT 

       310        320        330        340        350        360 
MPSLFGLTIT TWILSNISDK PLEPVEGKNR IKVYDGIYQS LAGQMSRVGI PSQRIPLALK 

       370        380        390        400        410        420 
DVSYLVEEVF KGKSPISGIS TRLTLTKWDP SKPISLQNVV VLTKNEQKVH EDRVLKGKES 

       430        440 
LQDVYDAKVL KLVSQRFREE AYYSQFR 

References and documentation are available.

Number of amino acids: 447

Molecular weight: 50265.76

Theoretical pI: 8.90

Amino acid composition: 
Ala (A)  21	  4.7%
Arg (R)  24	  5.4%
Asn (N)  18	  4.0%
Asp (D)  29	  6.5%
Cys (C)   5	  1.1%
Gln (Q)  20	  4.5%
Glu (E)  27	  6.0%
Gly (G)  27	  6.0%
His (H)   5	  1.1%
Ile (I)  28	  6.3%
Leu (L)  44	  9.8%
Lys (K)  39	  8.7%
Met (M)   5	  1.1%
Phe (F)  12	  2.7%
Pro (P)  20	  4.5%
Ser (S)  33	  7.4%
Thr (T)  27	  6.0%
Trp (W)   8	  1.8%
Tyr (Y)  12	  2.7%
Val (V)  43	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2246
Hydrogen    H	      3620
Nitrogen    N	       614
Oxygen      O	       670
Sulfur      S	        10

Formula: C2246H3620N614O670S10
Total number of atoms: 7160

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62130
Abs 0.1% (=1 g/l)   1.236, assuming all pairs of Cys residues form cystines


Ext. coefficient    61880
Abs 0.1% (=1 g/l)   1.231, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.02
This classifies the protein as stable.



Aliphatic index: 95.41

Grand average of hydropathicity (GRAVY): -0.333