ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVEPDMQKKA SGGSGGSEMD TLNATSNSSK QGVSNNKRNP VSKKKPGNKV SDGRDNAHNY 

        70         80         90        100        110        120 
HGEGRRKSSK QQRSRTPYKE TSTRINDQDI DLSIQEEILG GNFKLRGRKT QVSINHLLNF 

       130        140        150        160        170        180 
QLPEVEREKS RSSSSKKSNR RRDEHVHLHG DTFVNVNYRL LVDDRFDYPE QNCNPNVPVD 

       190        200        210        220        230        240 
QEKILRVIVP KGQNCSICLS EEPVAPRMVT CGHIFCLSCL LNFFSIEETV KNKETGYSKK 

       250        260        270        280        290        300 
KKYKECPLCG SIIGPKRVKP VLYEDDFDVT RLNQKPEPGA TVHLQLMCKP HGSLLPLPVA 

       310        320        330        340        350        360 
LHLDPLKCGN FPPANLGSIK HYAHIMKCGV SYSLELYQKD IVAIQEQYEI DKAIYNDSGK 

       370        380        390        400        410        420 
FVKQSIENIN DQISTLLAAT TDLSPLSNDI NNGLDNFHFD DDLLTKYDDS SAYFFYQTLV 

       430        440        450        460        470        480 
ASSTKYFLSP LDVKILLTIF HYYSKFPESI ETTVENIHYD TVVTEQLIRR YKYIGHLPIG 

       490        500        510        520        530        540 
TEIALLDLDW RKIPFLPKEI YEQFAHELKQ RRRKFTMKKQ KEDKEKKLYE KRLEQEHAEF 

       550        560        570        580        590        600 
YRKENGNSLK FEDSVQMATH YESIVSSSIP LNSLGISMLG PPTNSCSTPQ KQAPSHTKRT 

       610        620        630        640        650        660 
IWGTSIAVTE DEKASKENKE FQDMLLQRIR QEDSSDVTDS TDSPPTSNGK RGRKKKGKVM 

       670 
LFSSNHQALG 

References and documentation are available.

Number of amino acids: 670

Molecular weight: 76317.39

Theoretical pI: 8.75

Amino acid composition: 
Ala (A)  22	  3.3%
Arg (R)  33	  4.9%
Asn (N)  39	  5.8%
Asp (D)  41	  6.1%
Cys (C)  12	  1.8%
Gln (Q)  31	  4.6%
Glu (E)  46	  6.9%
Gly (G)  35	  5.2%
His (H)  21	  3.1%
Ile (I)  38	  5.7%
Leu (L)  58	  8.7%
Lys (K)  64	  9.6%
Met (M)  11	  1.6%
Phe (F)  24	  3.6%
Pro (P)  35	  5.2%
Ser (S)  63	  9.4%
Thr (T)  35	  5.2%
Trp (W)   2	  0.3%
Tyr (Y)  25	  3.7%
Val (V)  35	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3358
Hydrogen    H	      5333
Nitrogen    N	       947
Oxygen      O	      1038
Sulfur      S	        23

Formula: C3358H5333N947O1038S23
Total number of atoms: 10699

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49000
Abs 0.1% (=1 g/l)   0.642, assuming all pairs of Cys residues form cystines


Ext. coefficient    48250
Abs 0.1% (=1 g/l)   0.632, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.29
This classifies the protein as unstable.



Aliphatic index: 74.31

Grand average of hydropathicity (GRAVY): -0.743