ProtParam
User-provided sequence:
10 20 30 40 50 60
MVFDLKRIVR PKIYNLEPYR CARDDFTEGI LLDANENAHG PTPVELSKTN LHRYPDPHQL
70 80 90 100 110 120
EFKTAMTKYR NKTSSYANDP EVKPLTADNL CLGVGSDESI DAIIRACCVP GKEKILVLPP
130 140 150 160 170 180
TYSMYSVCAN INDIEVVQCP LTVSDGSFQM DTEAVLTILK NDSLIKLMFV TSPGNPTGAK
190 200 210 220 230 240
IKTSLIEKVL QNWDNGLVVV DEAYVDFCGG STAPLVTKYP NLVTLQTLSK SFGLAGIRLG
250 260 270 280 290 300
MTYATAELAR ILNAMKAPYN ISSLASEYAL KAVQDSNLKK MEATSKIINE EKMRLLKELT
310 320 330 340 350 360
ALDYVDDQYV GGLDANFLLI RINGGDNVLA KKLYYQLATQ SGVVVRFRGN ELGCSGCLRI
370 380
TVGTHEENTH LIKYFKETLY KLANE
References and
documentation are available.
Number of amino acids: 385
Molecular weight: 42646.12
Theoretical pI: 6.04
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1898
Hydrogen H 3043
Nitrogen N 499
Oxygen O 576
Sulfur S 18
Formula: C
1898H
3043N
499O
576S
18
Total number of atoms: 6034
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32820
Abs 0.1% (=1 g/l) 0.770, assuming all pairs of Cys residues form cystines
Ext. coefficient 32320
Abs 0.1% (=1 g/l) 0.758, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.19
This classifies the protein as stable.
Aliphatic index: 97.25
Grand average of hydropathicity (GRAVY): -0.127