ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVFSRDIPSP ASMFSTYASM MGYVMIIKPM INTIIPRPVQ NFVFSYLKSF AGSRSSTLTL 

        70         80         90        100        110        120 
TIDQMSSMYI PDELYAAAQA YLSTKISPNS VRLIMARDPA EKKVKLYLSD GEVVSDVYNG 

       130        140        150        160        170        180 
IKLKWRFLAR NKNNTMVEEY GQSYQGNIQR ESLELSFDKK HRDLVVNSYI PYVESKAKEV 

       190        200        210        220        230        240 
NNKRRILKMH CYSHMAQTWQ SVNFKHPSTF DTMAMNDDLK RSMIEDLDRF VGRKDFYKRV 

       250        260        270        280        290        300 
GKAWKRGYLL YGPPGTGKSS LVAAMANYLK FDIYDLQLAS VQGDAHLRSL LLATNNSSIL 

       310        320        330        340        350        360 
LIEDIDCSVD LPTRLQPPTE TSQPLGAVQV SKPLTLSGLL NCIDGLWSSC GNERIIIFTT 

       370        380        390        400        410        420 
NNKEKLDPAL LRPGRMDMHI YMGHCSFQGF KTLASNYLGL SDENDDTHPL CPDIKHLIDG 

       430        440        450        460        470        480 
HVLTPAQVAE ELMKDEDADA ALEGLVKVLK RKRLEPKKCD DESKMKKLKE GEEAIADAEL 

       490        500        510        520        530 
AVLTPAQVED KEELVASEYA NVMPEWLPRS RLVMVLPGFH RARGIRRGLG DEM 

References and documentation are available.

Number of amino acids: 533

Molecular weight: 60196.37

Theoretical pI: 7.59

Amino acid composition: 
Ala (A)  36	  6.8%
Arg (R)  29	  5.4%
Asn (N)  23	  4.3%
Asp (D)  36	  6.8%
Cys (C)   7	  1.3%
Gln (Q)  16	  3.0%
Glu (E)  31	  5.8%
Gly (G)  28	  5.3%
His (H)  11	  2.1%
Ile (I)  28	  5.3%
Leu (L)  58	 10.9%
Lys (K)  39	  7.3%
Met (M)  24	  4.5%
Phe (F)  16	  3.0%
Pro (P)  27	  5.1%
Ser (S)  44	  8.3%
Thr (T)  22	  4.1%
Trp (W)   5	  0.9%
Tyr (Y)  21	  3.9%
Val (V)  32	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      2669
Hydrogen    H	      4253
Nitrogen    N	       725
Oxygen      O	       794
Sulfur      S	        31

Formula: C2669H4253N725O794S31
Total number of atoms: 8472

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59165
Abs 0.1% (=1 g/l)   0.983, assuming all pairs of Cys residues form cystines


Ext. coefficient    58790
Abs 0.1% (=1 g/l)   0.977, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.51
This classifies the protein as unstable.



Aliphatic index: 87.09

Grand average of hydropathicity (GRAVY): -0.323