ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVFSYEHYMN LLFHLDNSKE TVPPEIAKRI ISNAIAPVIT VTSTPLFDKH IQETYKVDSL 

        70         80         90        100        110        120 
YMLLRFFGGC VSDRDQANEA KVGQHEHEVC DASDSTDSIP KNKNLEVPNL SKKGSRSRSN 

       130        140        150        160        170        180 
SLFQRDSTQS QYIRFTRPLG DLIETRDAND MLFNYHSLEV FLDNYLKLVA ANTDEMVPHN 

       190        200        210        220        230        240 
LLKKSIYHSF FSLAISSTNN LSPYETFNHP ILSLIALDIS NGEVYEDARD LLVNFKNLNH 

       250        260        270        280        290        300 
NTENFPIFMN TNEMLPVFLL CYNDDSQEEF EKCQALAKKL KKQLFVESIL LALWKDSFIY 

       310        320        330        340        350        360 
DENSVIQLHQ PVMSSLEEIL FFLQAPTQTT LSLALINSIY DMLDYLVYDL MIPFMKRKVS 

       370        380        390        400        410        420 
FWEETILQPR KSLFNGAKFF KKFMNKNPVN GNHQHNSLTR DSQGNEYFAS SSSEFLMRKL 

       430        440        450        460        470        480 
ADWSMMLSDF KTAYSTYESL MDDLDAFPKY LASCIEWCAV SLLMGAQSIV TVKMIKNDIN 

       490        500        510        520        530        540 
PLIERALATY ENCSRIQRGK GKESNSLDVT EPVRSYETRC MILASELFLS LSNTWTSTPY 

       550        560        570        580        590        600 
AIQYLETILD ECKLGPCSQI MVWERLSDCY NLRVDPRIKH RVGAMKKDAK DTEDLRGEHK 

       610        620        630        640        650        660 
YSTDHFTDED ILSEGLTRRR KAAFFRLIAA KKWAEQKQWR QVSWCLKDIE STYSEIKFLH 

       670        680        690 
GNGLILSKLK NQLNLKDVDS APRPSEKNLT RTSVSFIG 

References and documentation are available.

Number of amino acids: 698

Molecular weight: 80494.86

Theoretical pI: 6.00

Amino acid composition: 
Ala (A)  37	  5.3%
Arg (R)  31	  4.4%
Asn (N)  44	  6.3%
Asp (D)  44	  6.3%
Cys (C)  12	  1.7%
Gln (Q)  24	  3.4%
Glu (E)  46	  6.6%
Gly (G)  19	  2.7%
His (H)  17	  2.4%
Ile (I)  40	  5.7%
Leu (L)  81	 11.6%
Lys (K)  49	  7.0%
Met (M)  21	  3.0%
Phe (F)  37	  5.3%
Pro (P)  25	  3.6%
Ser (S)  68	  9.7%
Thr (T)  36	  5.2%
Trp (W)   9	  1.3%
Tyr (Y)  26	  3.7%
Val (V)  32	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 90
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3599
Hydrogen    H	      5613
Nitrogen    N	       951
Oxygen      O	      1077
Sulfur      S	        33

Formula: C3599H5613N951O1077S33
Total number of atoms: 11273

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    88990
Abs 0.1% (=1 g/l)   1.106, assuming all pairs of Cys residues form cystines


Ext. coefficient    88240
Abs 0.1% (=1 g/l)   1.096, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.43
This classifies the protein as unstable.



Aliphatic index: 86.20

Grand average of hydropathicity (GRAVY): -0.351