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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVFVKSTKSN AYFKRYQVKF RRRRDGKTDY RARIRLINQD KNKYNTPKYR FVVRFTNKDI

70 80 90 100 110 120
VAQIVSASIA GDIVKASAYA HELPQYGLTV GLTNYAAAYC TGLLLARRVL KMLEMDDEYE

130 140 150 160 170 180
GNVEATGEDF SVEPTDSRRP FRALLDVGLI RTTTGNRVFG ALKGALDGGL DIPHSDKRFA

190 200 210 220 230 240
GFHKENKQLD AEIHRNYIYG GHVSNYMKLL GEDEPEKLQT HFSAYIKKGV EAESIEELYK

250 260 270 280 290 300
KVHAAIRADP NPKKTVKPAP KQHKRYNLKK LTYEERKNKL IERVKALNGA GGDDDDEDDE


E


References and documentation are available.

Number of amino acids: 301 Molecular weight: 34357.98 Theoretical pI: 9.32
Amino acid composition: 
Ala (A) 26 8.6% Arg (R) 23 7.6% Asn (N) 15 5.0% Asp (D) 22 7.3% Cys (C) 1 0.3% Gln (Q) 7 2.3% Glu (E) 22 7.3% Gly (G) 21 7.0% His (H) 8 2.7% Ile (I) 14 4.7% Leu (L) 24 8.0% Lys (K) 31 10.3% Met (M) 4 1.3% Phe (F) 11 3.7% Pro (P) 10 3.3% Ser (S) 11 3.7% Thr (T) 15 5.0% Trp (W) 0 0.0% Tyr (Y) 17 5.6% Val (V) 19 6.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 44 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 1526 Hydrogen H 2421 Nitrogen N 439 Oxygen O 455 Sulfur S 5 Formula: C1526H2421N439O455S5 Total number of atoms: 4846 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 25330 Abs 0.1% (=1 g/l) 0.737, assuming all pairs of Cys residues form cystines Ext. coefficient 25330 Abs 0.1% (=1 g/l) 0.737, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 38.42 This classifies the protein as stable. Aliphatic index: 76.18 Grand average of hydropathicity (GRAVY): -0.748