ProtParam
User-provided sequence:
10 20 30 40 50 60
MVFVKSTKSN AYFKRYQVKF RRRRDGKTDY RARIRLINQD KNKYNTPKYR FVVRFTNKDI
70 80 90 100 110 120
VAQIVSASIA GDIVKASAYA HELPQYGLTV GLTNYAAAYC TGLLLARRVL KMLEMDDEYE
130 140 150 160 170 180
GNVEATGEDF SVEPTDSRRP FRALLDVGLI RTTTGNRVFG ALKGALDGGL DIPHSDKRFA
190 200 210 220 230 240
GFHKENKQLD AEIHRNYIYG GHVSNYMKLL GEDEPEKLQT HFSAYIKKGV EAESIEELYK
250 260 270 280 290 300
KVHAAIRADP NPKKTVKPAP KQHKRYNLKK LTYEERKNKL IERVKALNGA GGDDDDEDDE
E
References and
documentation are available.
Number of amino acids: 301
Molecular weight: 34357.98
Theoretical pI: 9.32
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 1526
Hydrogen H 2421
Nitrogen N 439
Oxygen O 455
Sulfur S 5
Formula: C
1526H
2421N
439O
455S
5
Total number of atoms: 4846
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25330
Abs 0.1% (=1 g/l) 0.737, assuming all pairs of Cys residues form cystines
Ext. coefficient 25330
Abs 0.1% (=1 g/l) 0.737, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.42
This classifies the protein as stable.
Aliphatic index: 76.18
Grand average of hydropathicity (GRAVY): -0.748