ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MVGSKDIDLF NLRENEQIVS PCLIVHGKCN KQNGAKTVQV QHPQLPPITY PIHNQFFKAT 

        70         80         90        100        110        120 
VILTPGENKL TFVTDTNTAR TIVCYYTPLT QNPPVHLCLI LAKDSPLQFD SPREQKDREG 

       130        140        150        160        170        180 
GNGLELAIKK LRLGARLMQA YTNEQMLRNS MGNRTFPFVE EFTWDTLFER PAMRNTIKIH 

       190        200        210        220        230        240 
VVRSEKTVKE IQDPDIAQQN SKGKNTGALF GIAMDALKSY GGPFTNNEKP VQAACMFLDT 

       250        260        270        280        290        300 
HWDGKLIRGH AALGGGDDSI KLAIFGSHGL YSWPTCLEQL VPYFTDETRS STSEVANDCN 

       310        320        330        340        350        360 
ECGTYWECLT ITLGAFMHEI GHLLGCPHQE SGVMLRGYTT LNRSFLTKEA YSVRTNSTGA 

       370        380        390        400        410        420 
SPPIFPKEEC TWNRLDTVRF LYHPSFTLPQ DYYDPSFMRP TKLGGYPNIK HSVYPLGNGS 

       430        440        450        460        470        480 
CRILSPTGIY LIEIICDDLA RGHIEYLPVS LGGQGPQREV IVTLDDLRAR LPKNELAKFG 

       490        500        510        520        530        540 
NTFKLKILSV NAPETEFDKF PSLLDVQPLD MSKYGFSKNV QGIKSPLYGR SDGGNAVGVV 

       550        560        570        580        590        600 
AFDVRLVTAV RIYHGYALDG VRFYYKEKPT GTKDAPASKP SVPPRNYFSK ITHSIKNHAS 

       610        620        630        640        650        660 
INEENLKSVL FGHETQNFTD ATLEPGEIII GFNLRCGAWV DAIQIITSHG RMTDMFGNKD 

       670        680        690 
GGGFAELQPP NGQYILGVTG RVGQWVDAFG IIYGAL 

References and documentation are available.

Number of amino acids: 696

Molecular weight: 77414.28

Theoretical pI: 7.26

Amino acid composition: 
Ala (A)  38	  5.5%
Arg (R)  32	  4.6%
Asn (N)  37	  5.3%
Asp (D)  35	  5.0%
Cys (C)  14	  2.0%
Gln (Q)  27	  3.9%
Glu (E)  36	  5.2%
Gly (G)  62	  8.9%
His (H)  19	  2.7%
Ile (I)  43	  6.2%
Leu (L)  63	  9.1%
Lys (K)  39	  5.6%
Met (M)  13	  1.9%
Phe (F)  34	  4.9%
Pro (P)  44	  6.3%
Ser (S)  38	  5.5%
Thr (T)  50	  7.2%
Trp (W)   7	  1.0%
Tyr (Y)  26	  3.7%
Val (V)  39	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      3470
Hydrogen    H	      5390
Nitrogen    N	       940
Oxygen      O	      1017
Sulfur      S	        27

Formula: C3470H5390N940O1017S27
Total number of atoms: 10844

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78115
Abs 0.1% (=1 g/l)   1.009, assuming all pairs of Cys residues form cystines


Ext. coefficient    77240
Abs 0.1% (=1 g/l)   0.998, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.95
This classifies the protein as stable.



Aliphatic index: 81.11

Grand average of hydropathicity (GRAVY): -0.302